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Potential roles of aquaporin 9 in the pathogenesis of endometriosis

 Young Sik Choi  ;  Ji Hyun Park  ;  Jeong-Kee Yoon  ;  Ji Sun Yoon  ;  Jung Sook Kim  ;  Jae Hoon Lee  ;  Bo Hyun Yun  ;  Joo Hyun Park  ;  Seok Kyo Seo  ;  SiHyun Cho  ;  Byung Seok Lee  ;  Hugh S.Taylor 
 Molecular Human Reproduction, Vol.25(7) : 373-384, 2019 
Journal Title
Issue Date
AQP9 ; aquaporins ; endometriosis ; extracellular signal-regulated kinase 1/2 ; matrix metalloproteinase ; migration ; p38 mitogen-activated protein kinases ; pathogenesis ; siRNA ; transfection
Aquaporins (AQPs) are involved in cell migration, proliferation and carcinogenesis in tumor development and physiologic inflammatory processes, but their associations with endometriosis have not been fully evaluated. In this study, tissue samples were obtained from women undergoing laparoscopic surgery for endometriosis and other benign conditions. Analysis of expressions of AQP subtypes in eutopic and ectopic endometrium of patients with endometriosis (Eu-EMS and Ect-EMS, respectively) and eutopic endometrium of control patients without endometriosis (Eu-CTL) were performed using the NanoString nCounter System and western blotting. Human endometrial stromal cells (HESCs) were cultured and transfected with the siRNA of the AQP of interest. Among the AQP1-9 subtypes, endometrial expression of AQP2 and AQP8 was significantly increased, whereas AQP9 expression was significantly decreased in the Eu-EMS group compared to the Eu-CTL group. Comparison of expression of AQP2, AQP8 and AQP9 among Eu-EMS, Ect-EMS and Eu-CTL groups revealed significant differences for only AQP9. Expression of AQP9 in the Eu-EMS group was decreased compared with that in Eu-CTL. After transfection of AQP9 siRNA in HESCs, expressions of MMP2 and MMP9 were significantly elevated. Increased expression of phosphorylated ERK 1/2 and phosphorylated p38 MAPK proteins after transfection was also confirmed using western blot analysis. Increased migration and invasion potentials of HESCs after transfection were determined by migration and wound healing assays. These findings suggest that AQP9 may be involved in the pathogenesis of endometriosis and warrant further investigation as a potential therapeutic target for treating endometriosis.
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1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Jeong Sook(김정숙)
Park, Joo Hyun(박주현)
Seo, Seok Kyo(서석교) ORCID logo https://orcid.org/0000-0003-3404-0484
Yun, Bo Hyon(윤보현) ORCID logo https://orcid.org/0000-0001-5703-797X
Yun, Jisun(윤지선)
Lee, Byung Seok(이병석) ORCID logo https://orcid.org/0000-0001-6001-2079
Lee, Jae Hoon(이재훈) ORCID logo https://orcid.org/0000-0003-4223-1395
Cho, Si Hyun(조시현) ORCID logo https://orcid.org/0000-0003-2718-6645
Choi, Young Sik(최영식) ORCID logo https://orcid.org/0000-0002-1157-4822
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