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후천성면역결핍증 바이러스 항원 및 항체 동시 선별검사의 진단적 유용성

Other Titles
 Evaluation of a Screening Test Kit of Simultaneous p24 Antigen and Anti-HIV1/2 Detection in Diagnostic Aspect 
 박광일  ;  김현숙  ;  김현옥  ;  송경순  ;  김준명 
 Korean Journal of Clinical Pathology (대한임상병리학회지), Vol.20(3) : 330-336, 2000 
Journal Title
 Korean Journal of Clinical Pathology (대한임상병리학회지) 
Issue Date
AIDS ; HIV ; HIV screening test ; Anti-HIV1/2 antibody ; p24 antigen
Background : Human immunodeficiency virus (HIV) is the causative agent of acquired immunodeficiency syndrome (AIDS). Current diagnosis of HIV infection relies on the detection of anti-HIV antibodies by ELISA. Recently, simultaneous detection kit of p24 antigenemia and anti-HIV1/anti-HIV2 antibodies was developed. The aim of this study was to evaluate the diagnostic kit of simultaneous detection with p24 antigen and anti-HIV1/2 in diagnostic aspect. Methods : Eight hundred and four sera which were obtained between July 1999 and August 1999 and 110 sera from 54 patients diagnosed with HIV infection were included. One lot of panels composed of consecutive sera obtained from known HIV-infected patients was included. The detection of anti-HIV1/2 antibodies was done by Genedia HIV1/2 ELISA 3.0 kit (Greencross, Seoul,Korea) and Enzygnost anti-HIV1/2 Plus (Behringwerke, Marburg, Germany). The simultaneous detection of p24 antigenemia and anti-HIV1/2 antibodies was done with VIDAS DUO kit (bioMerieux, Lyon, France). The Vironostika HIV-1 antigen kit was used for detection of p24 antigen. The HIV RNA PCR and anti-HIV western blot assay were also performed to confirm the test results in discrepant cases. Results : The simultaneous detection kit showed 100% sensitivity and 99.6% specificity. The possibility of obtaining an earlier diagnosis than from the conventional anti-HIV1/2 EIA was also suggested by the results obtained with a group of consecutive panel sera infected with HIV. Conclusion : The simultaneous p24 antigen and anti-HIV1/2 detection kit can be applied as a clinical screening test as a substitution for conventional anti-HIV1/2 EIA, with a probable gain, especially, in early diagnosis. (Korean J Clin Pathol 2000; 20: 330-6)
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1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyon Suk(김현숙) ORCID logo https://orcid.org/0000-0001-5662-7740
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