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불임 환자에 있어서 황체형성호르몬에 관한 유전자 분석

Other Titles
 LH-B gene analysis in infertility patients 
 김정연  ;  박기현  ;  배상욱  ;  이병석  ;  안용호 
 Korean Journal of Obstetrics and Gynecology (대한산부인과학회잡지), Vol.43(8) : 1389-1393, 2000 
Journal Title
Korean Journal of Obstetrics and Gynecology(대한산부인과학회잡지)
Issue Date
PCR RFLP ; Infertility ; LH-βgene mutation ; PCR ; LH-β Subunit mutation ; PCR
Objectives : To investigate the presence of LH-β gene mutaion in women with infertility by analyzing the gene sequence of LH-β subunit. Materials and Methods : From June 1999 to March 2000, nuclear DNA was extracted from peripheral blood samples of 32 women infertility patients and the exon 3 of LH-β subunit gene was replicated by PCR(Polymerase Chain Reaction). The product of PCR was analyzed for the presence of point mutation from G1502 to A1502 within the LH-βsubunit gene by RFLP(Restriction Fragment Length Polymorphism). Additionally Direct PCR mediated DNA sequencing was performed. Result : In the 32 infertility women studied, the point mutation from G1502 to A1502 was not present and 2 normal bands were observed on RFLP. Conclusion : The purpose of this study was to investigate whether a point mutation from G1502 to A1502 is present in women with infertility. Very few cases of LH-β gene mutation have been reported, and since the site of point mutation is highly variable, identifying and localzing LH-β gene mutation should be difficult and further studies must be conducted
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers
Yonsei Authors
Bai, Sang Wook(배상욱) ORCID logo https://orcid.org/0000-0001-7724-7552
Lee, Byung Seok(이병석) ORCID logo https://orcid.org/0000-0001-6001-2079
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