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임상 검체에서 결핵균 검출을 위한 항산성염색, PCR, LCR, PCR-Hybridization 검사법 간의 비교

Other Titles
 Comparison of Acid-Fast staining, PCR, LCR, PCR=Hybridization for dection of mycobacterum tuberculosis in clinical specimens 
Authors
 Jong Rak Choi  ;  Jong Baeck Lim  ;  Hyung Jung Kim 
Citation
 Tuberculosis and Respiratory Diseases (결핵 및 호흡기질환), Vol.49(3) : 281-289, 2000 
Journal Title
TUBERCULOSIS AND RESPIRATORY DISEASES(결핵 및 호흡기질환)
ISSN
 0378-006 
Issue Date
2000
MeSH
Mycobacterium tuberculosis ; Polymerase Chain Reaction* ; Tuberculosis*
Keywords
Mycobacterium tuberculosis ; Culture ; PCR ; Ligase chain reaction(LCR) ; PCR-Hybridization
Abstract
Background

Mycobacterial culture is a confirmatory test to detect M.tuberculosis, but it takes at least 6 weeks to diagnose. PCR is a rapid and sensitive method, but it is known that PCR has a high false positive rate due to contamination, and a high false negative rate due to inhibitors. It is also known that LCR and PCR-Hybridization, recently developed methods, are more specific methods than PCR in terms of detection M.tuberculosis. In this study, we estimated the clinical utility of in house PCR, LCR and PCR-Hybridization for the detection of M.tuberculosis.


Methods

We evaluated 75 specimens, upon which M.tuberculosis culture based testing was requested, by PCR LCR, and PCR-Hybridization and compared results. Mycobacterial culture was performed on 3% Ogawa media for 8 weeks, and an in house PCR, LCx Mycobacterium tuberculosis assay kit(Abbott Laboratories, North Chicago, III) and the AMPLICOR M.tuberculosis test kit(Roche Molecular Systems, Inc. Branchburg, NJ, USA).


Results

In the view of the culture results, the sensitivities of the three tests were 40%, 80%, and 100% and their specificities were 98.6%, 94.3%, and 94.3%.


Conclusion

LCR and PCR-Hybridization and rapid and sensitive methods for detecting M.tuberculosis in clinical laboratories.
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyung Jung(김형중) ORCID logo https://orcid.org/0000-0003-2498-0683
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/171962
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