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임상 검체에서 결핵균 검출을 위한 항산성염색, PCR, LCR, PCR-Hybridization 검사법 간의 비교

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dc.contributor.author김형중-
dc.date.accessioned2019-11-11T05:32:37Z-
dc.date.available2019-11-11T05:32:37Z-
dc.date.issued2000-
dc.identifier.issn0378-006-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/171962-
dc.description.abstractBackground Mycobacterial culture is a confirmatory test to detect M.tuberculosis, but it takes at least 6 weeks to diagnose. PCR is a rapid and sensitive method, but it is known that PCR has a high false positive rate due to contamination, and a high false negative rate due to inhibitors. It is also known that LCR and PCR-Hybridization, recently developed methods, are more specific methods than PCR in terms of detection M.tuberculosis. In this study, we estimated the clinical utility of in house PCR, LCR and PCR-Hybridization for the detection of M.tuberculosis. Methods We evaluated 75 specimens, upon which M.tuberculosis culture based testing was requested, by PCR LCR, and PCR-Hybridization and compared results. Mycobacterial culture was performed on 3% Ogawa media for 8 weeks, and an in house PCR, LCx Mycobacterium tuberculosis assay kit(Abbott Laboratories, North Chicago, III) and the AMPLICOR M.tuberculosis test kit(Roche Molecular Systems, Inc. Branchburg, NJ, USA). Results In the view of the culture results, the sensitivities of the three tests were 40%, 80%, and 100% and their specificities were 98.6%, 94.3%, and 94.3%. Conclusion LCR and PCR-Hybridization and rapid and sensitive methods for detecting M.tuberculosis in clinical laboratories.-
dc.description.statementOfResponsibilityopen-
dc.languageKorean-
dc.publisher대한결핵 및 호흡기학회-
dc.relation.isPartOfTuberculosis and Respiratory Diseases (결핵 및 호흡기질환)-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.subject.MESHMycobacterium tuberculosis-
dc.subject.MESHPolymerase Chain Reaction*-
dc.subject.MESHTuberculosis*-
dc.title임상 검체에서 결핵균 검출을 위한 항산성염색, PCR, LCR, PCR-Hybridization 검사법 간의 비교-
dc.title.alternativeComparison of Acid-Fast staining, PCR, LCR, PCR=Hybridization for dection of mycobacterum tuberculosis in clinical specimens-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학교실)-
dc.contributor.googleauthorJong Rak Choi-
dc.contributor.googleauthorJong Baeck Lim-
dc.contributor.googleauthorHyung Jung Kim-
dc.contributor.localIdA01158-
dc.relation.journalcodeJ02762-
dc.subject.keywordMycobacterium tuberculosis-
dc.subject.keywordCulture-
dc.subject.keywordPCR-
dc.subject.keywordLigase chain reaction(LCR)-
dc.subject.keywordPCR-Hybridization-
dc.contributor.alternativeNameKim, Hyung Jung-
dc.contributor.affiliatedAuthor김형중-
dc.citation.volume49-
dc.citation.number3-
dc.citation.startPage281-
dc.citation.endPage289-
dc.identifier.bibliographicCitationTuberculosis and Respiratory Diseases (결핵 및 호흡기질환), Vol.49(3) : 281-289, 2000-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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