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The role of lipid peroxidation and glutathione on the glycochenodeoxycholic acid-induced cell death in primary cultured rat hepatocytes

Authors
 SangHui Chu  ;  WolMi Park  ;  KyungEun Lee  ;  YoungSook Pae 
Citation
 Korean Journal of Physiology & Pharmacology, Vol.4(2) : 121-127, 2000 
Journal Title
KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY
ISSN
 1226-4512 
Issue Date
2000
Keywords
Hepatocyte ; Apoptosis ; Glycochenodeoxycholic acid ; Lipid peroxidation ; Glutathione
Abstract
Intracellular accumulation of bile acids in the hepatocytes during cholestasis is thought to be pathogenic in cholestatic liver diseases. The objective of this study was to determine the role of lipid peroxidation and glutathione on the bile acid-induced hepatic cell death mechanism in primary cultured rat hepatocytes. To induce hepatic cell death, we incubated primary cultured rat hepatocytes with glycochenodeoxycholic acid (GCDC; 0∼400μM) for 3 hours. In electron microscopic examination and agarose gel electrophoresis, low concentration of GCDC treatment mainly induced apoptotic feature. Whereas 400μM GCDC treated cells demonstrated both apoptosis and necrosis. Lipid peroxidation was increased dose- dependently in GCDC treated hepatocyte. And this was also accompanied by decreased glutathione. Therefore, oxygen free radical damage may play a partial role in GCDC-induced hepatic cell death.
Files in This Item:
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Appears in Collections:
3. College of Nursing (간호대학) > Dept. of Nursing (간호학과) > 1. Journal Papers
Yonsei Authors
Chu, Sang Hui(추상희) ORCID logo https://orcid.org/0000-0001-6877-5599
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/171789
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