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Genotyping of Giardia lamblia isolates from humans in China and Korea using ribosomal DNA sequences

Authors
 T-S. Yong  ;  S-J. Park  ;  U-W. Hwang  ;  H-W. Yang  ;  K-W. Lee  ;  D-Y. Min  ;  H-J. Rim  ;  Y. Wang  ;  F. Zheng 
Citation
 Journal of Parasitology, Vol.86(4) : 887-891, 2000 
Journal Title
JOURNAL OF PARASITOLOGY
ISSN
 0022-3395 
Issue Date
2000
MeSH
Animals ; Base Sequence ; China ; DNA, Protozoan/chemistry ; DNA, Ribosomal/chemistry* ; Dogs ; Genotype ; Giardia lamblia/classification ; Giardia lamblia/genetics* ; Giardiasis/parasitology* ; Humans ; Korea ; Molecular Sequence Data ; Phylogeny ; Polymerase Chain Reaction ; RNA, Protozoan/genetics ; RNA, Ribosomal, 16S/genetics* ; Rodentia ; Sequence Alignment ; Sequence Analysis, DNA
Abstract
Genetic characterization of a total of 15 Giardia lamblia isolates, 8 from Anhui Province, China (all from purified cysts) and 7 from Seoul, Korea (2 from axenic cultures and 5 from purified cysts), was performed by polymerase chain reaction amplification and sequencing of a 295-bp region near the 5' end of the small subunit ribosomal DNA (eukaryotic 16S rDNA). Phylogenetic analyses were subsequently conducted using sequence data obtained in this study, as well as sequences published from other Giardia isolates. The maximum parsimony method revealed that G. lamblia isolates from humans in China and Korea are divided into 2 major lineages, assemblages A and B. All 7 Korean isolates were grouped into assemblage A, whereas 4 Chinese isolates were grouped into assemblage A and 4 into assemblage B. Two Giardia microti isolates and 2 dog-derived Giardia isolates also grouped into assemblage B, whereas Giardia ardeae and Giardia muris were unique.
Full Text
http://www.bioone.org/doi/10.1645/0022-3395(2000)086[0887:GOGLIF]2.0.CO;2
DOI
10.1645/0022-3395(2000)086[0887:GOGLIF]2.0.CO;2
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Tropica Medicine (열대의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Park, Soon Jung(박순정) ORCID logo https://orcid.org/0000-0002-0423-1944
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/171732
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