32 66

Cited 0 times in

Variable numbers of TTC repeats in Mycobacterium leprae DNA from leprosy patients and use in strain differentiation.

Authors
 YOO-CHUL SHIN  ;  HYEJON LEE  ;  HYEYOUNG LEE  ;  GERALD P. WALSH  ;  JOO-DEUK KIM  ;  SANG-NAE CHO 
Citation
 Journal of Clinical Microbiology, Vol.38(12) : 4535-4538, 2000 
Journal Title
 Journal of Clinical Microbiology 
ISSN
 0095-1137 
Issue Date
2000
MeSH
Autoradiography ; DNA, Bacterial/chemistry* ; Humans ; Leprosy/diagnosis* ; Leprosy/microbiology ; Mycobacterium leprae/classification* ; Mycobacterium leprae/genetics ; Polymerase Chain Reaction ; Reproducibility of Results ; Trinucleotide Repeats*
Abstract
Strain differentiation of Mycobacterium leprae would be of great value for epidemiological investigation to identify the infectious sources of leprosy, to understand transmission patterns, and to distinguish between relapse and reinfection. From the M. leprae genome sequence database, TTC DNA repeats were identified. Primer sets designed to amplify the region flanking TTC repeats revealed PCR products of different sizes, indicating that the number of repeats at each locus may be variable among M. leprae strains. The TTC repeats were not found in Mycobacterium tuberculosis, Mycobacterium avium, Mycobacterium marinum, or human tissues, which indicated their specificity to M. leprae. Sequence analysis of the TTC repeat region in each of the M. leprae strains showed a variation of 10 to 37 repeats. In the M. leprae strains of 34 multibacillary patients at Cebu, Philippines, M. leprae with 24 and 25 TTC repeats was most common, and this was followed by strains with 14, 15, 20, 21, and 28 repeats. This study thus indicates that there are variable numbers of TTC repeats in a noncoding region of M. leprae strains and that the TTC region may be useful for strain differentiation for epidemiological investigations of leprosy.
Files in This Item:
T200002569.pdf Download
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Cho, Sang Nae(조상래)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/171689
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links