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Serodiagnosis of amoebiasis using a recombinant protein fragment of the 29 kDa surface antigen of Entamoeba histolytica

Authors
 Jongweon Lee  ;  Soon-Jung Park  ;  Tai-Soon Yong 
Citation
 International Journal for Parasiology, Vol.30(14) : 1487-1491, 2000 
Journal Title
 International Journal for Parasiology 
ISSN
 0020-7519 
Issue Date
2000
MeSH
Amebiasis/diagnosis* ; Amino Acid Sequence ; Animals ; Antigens, Surface/immunology* ; Entamoeba histolytica/immunology* ; Enzyme-Linked Immunosorbent Assay ; Humans ; Immunodominant Epitopes/chemistry ; Molecular Sequence Data ; Peptide Fragments ; Recombinant Proteins/immunology* ; Sensitivity and Specificity ; Sequence Alignment
Keywords
Enzyme-linked immunosorbent assay ; Entamoeba histolytica ; Immunodominant epitopes ; Serodiagnosis
Abstract
To develop an improved serodiagnostic test for amoebiasis, we performed a detailed analysis of the immunodominant epitopes of the 29 kDa surface antigen and evaluated its sensitivity and specificity. Enzyme-linked immunosorbent assay (ELISA) based on the fragment containing the immunodominant epitope was evaluated further and compared with full-length recombinant 29 kDa protein. Specificity and sensitivity of the two ELISAs were assessed using 55 human sera of parasitic protozoa infection cases (25 amoebiasis, 20 giardiasis and 10 toxoplasmosis sera) and 10 healthy control sera. The immunodominant epitope of the 29 kDa antigen is localised only in the N-terminus 14-54 amino acid residues. The sensitivities of the two ELISAs were very high, 92 and 96%, respectively. The specificity of the fragment was 100%, whereas the specificity of the full-length 29 kDa protein was 86.6%. These results indicate that the fragment containing the immunodominant epitope of the 29 kDa protein can be used to accurately serodiagnose amoebiasis without cross-reactivity from other parasites.
Full Text
https://www.sciencedirect.com/science/article/pii/S0020751900001120
DOI
10.1016/S0020-7519(00)00112-0
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Park, Soon Jung(박순정) ORCID logo https://orcid.org/0000-0002-0423-1944
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/171655
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