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Differential effects of retinoids on the growth and apoptosis in human colon cancer cell lines associated with the induction of RARβ

Authors
 Mi-Ock Lee  ;  Sun-Young Han  ;  Shunai Jiang  ;  Jeon Han Park  ;  Se Jong Kim 
Citation
 International Journal for Parasiology, Vol.59(5) : 485-496, 2000 
Journal Title
 International Journal for Parasiology 
ISSN
 0020-7519 
Issue Date
2000
MeSH
Antineoplastic Agents/pharmacology* ; Apoptosis* ; Cell Division/drug effects ; Colonic Neoplasms ; Gene Expression Regulation, Neoplastic ; Humans ; Proto-Oncogene Proteins/biosynthesis ; Proto-Oncogene Proteins c-bcl-2/biosynthesis ; Proto-Oncogene Proteins c-myc/biosynthesis ; Receptors, Retinoic Acid/biosynthesis* ; Receptors, Retinoic Acid/genetics ; Retinoic Acid Receptor alpha ; Transfection ; Tretinoin/pharmacology* ; Tumor Cells, Cultured ; bcl-2-Associated X Protein
Abstract
Retinoids are well known as potential chemopreventive and chemotherapeutic agents against a variety of human cancers. Here, we report that retinoic acid (RA) induced differential growth inhibition in human colon cancer cell lines: while DLD-1, HT-29, and WiDr were relatively resistant, HCT-15 and Colo201 were relatively sensitive. All-trans-retinoic acid caused morphological and biochemical changes such as membrane shrinkage, chromatin condensation, and DNA cleavage, which are typical features of cells undergoing apoptosis in sensitive cell lines. Although retinoic acid receptor (RAR)alpha, beta, gamma and retinoid X receptor alpha were expressed in all cell lines examined, a significant induction of RARbeta by all-trans-RA was observed only in sensitive cell lines, suggesting important roles of RARbeta in RA sensitivity. When a vector containing the RARbeta gene was introduced into a relatively resistant cell line, DLD-1, the cells acquired RA sensitivity. Further, we found that the RARbeta transfectants of DLD-1 expressed an enhanced level of c-Myc and Bax proteins, which may result in the increased susceptibility of the cells to all-trans-RA-induced apoptosis. In summary, our data demonstrated that RA induced growth inhibition and apoptosis in human colon cancer cells and that the induction of RAR3 may mediate the retinoid action.
Full Text
https://www.sciencedirect.com/science/article/pii/S000629529900355X
DOI
10.1016/S0006-2952(99)00355-X
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Se Jong(김세종)
Park, Jeon Han(박전한) ORCID logo https://orcid.org/0000-0001-9604-3205
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/171654
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