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Inhibition of MAP kinase by a Drosophila dual-specific phosphatase.

 Won-Jae LEE  ;  Sun-Hong KIM  ;  Yong-Sik KIM  ;  Sung-Jun HAN  ;  Ki-Sook PARK  ;  Ji-Hwan RYU  ;  Man-Wook HUR  ;  Kang-Yell CHOI 
 Biochemical Journal, Vol.349(3) : 821-828, 2000 
Journal Title
Issue Date
Amino Acid Sequence ; Animals ; Base Sequence ; DNA Primers ; Down-Regulation ; Drosophila/enzymology* ; Drosophila Proteins ; Dual Specificity Phosphatase 1 ; Mitogen-Activated Protein Kinases/antagonists & inhibitors* ; Molecular Sequence Data ; Pheromones/metabolism ; Protein Phosphatase 1 ; Protein Tyrosine Phosphatases/chemistry ; Protein Tyrosine Phosphatases/genetics ; Protein Tyrosine Phosphatases/metabolism* ; Saccharomyces cerevisiae/metabolism ; Sequence Homology, Amino Acid
Drosophila development ; MAP kinase ; protein phosphatases ; signal transduction
The Drosophila extracellular signal-regulated kinase (DERK) mitogen-activated protein kinase (MAPK) is involved in the regulation of multiple differentiation and developmental processes. Tight control of MAPK activity is critical for normal cell behaviour. We identified a novel Drosophila MAPK phosphatase (DMKP) cDNA from the expressed-sequence-tag database and characterized it. Analysis of the nucleotide sequence revealed an open reading frame encoding the 203-amino acid protein, with a calculated molecular mass of 23 kDa, which has a high amino acid sequence similarity with 'VH1-like' dual-specific phosphatases at the broad region near the catalytic sites. The expression of DMKP mRNA occurs from the late larval stages to adulthood in Drosophila development. The recombinant DMKP protein produced in yeast retained its phosphatase activity. When expressed in Schneider cells, DMKP dose-dependently inhibited DERK and Drosophila c-Jun N-terminal kinase activities with high selectivity towards DERK. However, DMKP did not have any affect on Drosophila p38 activity. When DMKP was expressed in yeast, it down-regulated the fus1-lacZ trans-reporter gene of the pheromone MAPK pathway without any significant effect on the high-osmolarity-glycerol-response pathway.
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1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
Hur, Man Wook(허만욱) ORCID logo https://orcid.org/0000-0002-3416-1334
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