0 88

Cited 12 times in

Molecular Characterization of Key Diphtheria Toxin:Receptor (proHB-EGF)Interactions.

Authors
 Joanna S. Brooke  ;  Jeong-Heon Cha 
Citation
 Biochemical and Biophysical Research Communications, Vol.275(2) : 374-381, 2000 
Journal Title
 Biochemical and Biophysical Research Communications 
ISSN
 0006-291X 
Issue Date
2000
MeSH
Animals ; Cell Line ; Diphtheria Toxin/chemistry ; Diphtheria Toxin/genetics ; Diphtheria Toxin/metabolism* ; Heparin-binding EGF-like Growth Factor ; Intercellular Signaling Peptides and Proteins ; Lysine/chemistry ; Lysine/metabolism ; Mutagenesis, Site-Directed ; Protein Binding ; Receptors, Cell Surface/genetics ; Receptors, Cell Surface/metabolism*
Keywords
diphtheria toxin ; diphtheria toxin receptor ; HB-EGF ; toxin:receptor interaction
Abstract
The major amino acids necessary for diphtheria toxin (DT) binding to its receptor have been identified previously. Studies by W. H. Shen et al. (J. Biol. Chem. 269, 29077-29084, 1994) and by J. H. Cha et al. (Mol. Microbiol. 29 (5), 1275-1284, 1998) suggested that the positively charged nature of the single amino acid residue, (516)Lys of DT, is crucial for binding to the DT receptor, whereas the negatively charged (141)Glu of the DT receptor is the most important residue for toxin binding. Here, we hypothesize that key interactions occur between these two oppositely charged amino acid residues. Reciprocal substitution of the residues at these positions between the toxin and the receptor was performed, which resulted in a partial reconstitution of the toxin:receptor interaction. This study provides the first biological data that characterizes the specific interaction of these two key residues with each other and also the additional interactions between other positively charged residues of DT and (141)Glu of the DT receptor.
Full Text
https://www.sciencedirect.com/science/article/pii/S0006291X00933175
DOI
10.1006/bbrc.2000.3317
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Cha, Jeong Heon(차정헌) ORCID logo https://orcid.org/0000-0002-9385-2653
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/171567
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links