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Secretory differentiation of serially passaged normal human nasal epithelial cells by retinoic acid: expression of mucin and lysozyme.

Authors
 Joo-Heon Yoon  ;  Kyung-Su Kim  ;  Jeung-Gweon Lee  ;  Sung-Shik Kim  ;  In Yong Park 
Citation
 Annals of Otology, Rhinology & Laryngology, Vol.109(6) : 594-601, 2000 
Journal Title
 Annals of Otology, Rhinology & Laryngology 
ISSN
 0003-4894 
Issue Date
2000
MeSH
Cell Differentiation/drug effects* ; Cells, Cultured ; Epithelial Cells/drug effects ; Epithelial Cells/secretion ; Gene Expression Regulation, Enzymologic/drug effects ; Humans ; Mucins/genetics* ; Muramidase/genetics* ; Nasal Mucosa/drug effects* ; Nasal Mucosa/secretion ; RNA, Messenger/genetics ; Reverse Transcriptase Polymerase Chain Reaction ; Secretory Rate/drug effects* ; Tretinoin/pharmacology*
Keywords
lysozyme ; mucin ; normal human nasal epithelial cells ; retinoic acid ; subculture
Abstract
The purpose of this study was to subculture normal human nasal epithelial (NHNE) cells without compromising their ability to differentiate into secretory and ciliated cells and to study the effect of retinoic acid on mucous and serous secretions in passaged cells and to compare the expression of mucin and lysozyme in cultured cells with those in in vivo nasal epithelium. The subcultured cells were tested after every passage for secretory differentiation in air-liquid interface cultures. The cultured NHNE cells secreted mucin and lysozyme. The cells became squamous and mucin secretion decreased when retinoic acid was deleted from the culture media. Cells from passage 1 through passage 2 remained able to differentiate into mucous or squamous cells. Mucin gene 4 (MUC4), MUC5AC, MUC7, MUC8, and lysozyme messenger RNAs were expressed in passage 2 NHNE cells. In conclusion, passage 2 NHNE cell cultures retain features of normal epithelium and are suitable for many studies of upper airway cell biology.
Full Text
http://journals.sagepub.com/doi/abs/10.1177/000348940010900612
DOI
10.1177/000348940010900612
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Su(김경수) ORCID logo https://orcid.org/0000-0003-1460-0640
Yoon, Joo Heon(윤주헌)
Lee, Jeung Gweon(이정권)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/171547
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