Cited 74 times in
Secretory differentiation of serially passaged normal human nasal epithelial cells by retinoic acid: expression of mucin and lysozyme.
DC Field | Value | Language |
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dc.contributor.author | 김경수 | - |
dc.contributor.author | 윤주헌 | - |
dc.contributor.author | 이정권 | - |
dc.date.accessioned | 2019-11-11T05:01:19Z | - |
dc.date.available | 2019-11-11T05:01:19Z | - |
dc.date.issued | 2000 | - |
dc.identifier.issn | 0003-4894 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/171547 | - |
dc.description.abstract | The purpose of this study was to subculture normal human nasal epithelial (NHNE) cells without compromising their ability to differentiate into secretory and ciliated cells and to study the effect of retinoic acid on mucous and serous secretions in passaged cells and to compare the expression of mucin and lysozyme in cultured cells with those in in vivo nasal epithelium. The subcultured cells were tested after every passage for secretory differentiation in air-liquid interface cultures. The cultured NHNE cells secreted mucin and lysozyme. The cells became squamous and mucin secretion decreased when retinoic acid was deleted from the culture media. Cells from passage 1 through passage 2 remained able to differentiate into mucous or squamous cells. Mucin gene 4 (MUC4), MUC5AC, MUC7, MUC8, and lysozyme messenger RNAs were expressed in passage 2 NHNE cells. In conclusion, passage 2 NHNE cell cultures retain features of normal epithelium and are suitable for many studies of upper airway cell biology. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Sage | - |
dc.relation.isPartOf | Annals of Otology, Rhinology & Laryngology | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Cell Differentiation/drug effects* | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Epithelial Cells/drug effects | - |
dc.subject.MESH | Epithelial Cells/secretion | - |
dc.subject.MESH | Gene Expression Regulation, Enzymologic/drug effects | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Mucins/genetics* | - |
dc.subject.MESH | Muramidase/genetics* | - |
dc.subject.MESH | Nasal Mucosa/drug effects* | - |
dc.subject.MESH | Nasal Mucosa/secretion | - |
dc.subject.MESH | RNA, Messenger/genetics | - |
dc.subject.MESH | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.subject.MESH | Secretory Rate/drug effects* | - |
dc.subject.MESH | Tretinoin/pharmacology* | - |
dc.title | Secretory differentiation of serially passaged normal human nasal epithelial cells by retinoic acid: expression of mucin and lysozyme. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Otorhinolaryngology (이비인후과학교실) | - |
dc.contributor.googleauthor | Joo-Heon Yoon | - |
dc.contributor.googleauthor | Kyung-Su Kim | - |
dc.contributor.googleauthor | Jeung-Gweon Lee | - |
dc.contributor.googleauthor | Sung-Shik Kim | - |
dc.contributor.googleauthor | In Yong Park | - |
dc.identifier.doi | 10.1177/000348940010900612 | - |
dc.contributor.localId | A00298 | - |
dc.contributor.localId | A02604 | - |
dc.contributor.localId | A03095 | - |
dc.relation.journalcode | J00173 | - |
dc.identifier.eissn | 1943-572X | - |
dc.identifier.pmid | 10855573 | - |
dc.identifier.url | http://journals.sagepub.com/doi/abs/10.1177/000348940010900612 | - |
dc.subject.keyword | lysozyme | - |
dc.subject.keyword | mucin | - |
dc.subject.keyword | normal human nasal epithelial cells | - |
dc.subject.keyword | retinoic acid | - |
dc.subject.keyword | subculture | - |
dc.contributor.alternativeName | Kim, Kyung Su | - |
dc.contributor.affiliatedAuthor | 김경수 | - |
dc.contributor.affiliatedAuthor | 윤주헌 | - |
dc.contributor.affiliatedAuthor | 이정권 | - |
dc.citation.volume | 109 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 594 | - |
dc.citation.endPage | 601 | - |
dc.identifier.bibliographicCitation | Annals of Otology, Rhinology & Laryngology, Vol.109(6) : 594-601, 2000 | - |
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