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Commensal-derived metabolites govern Vibrio cholerae pathogenesis in host intestine

Authors
 Jin Sun You  ;  Ji Hyun Yong  ;  Gwang Hee Kim  ;  Sungmin Moon  ;  Ki Taek Nam  ;  Ji Hwan Ryu  ;  Mi Young Yoon  ;  Sang Sun Yoon 
Citation
 Microbiome, Vol.7(1) : e132, 2019 
Journal Title
 Microbiome 
Issue Date
2019
Keywords
Amino sugars ; Bacteroides vulgatus ; Clindamycin ; Colonization resistance ; Gut microbiota ; Metabolomics ; Short-chain fatty acids ; Vibrio cholerae
Abstract
BACKGROUND: Recent evidence suggests that the commensal microbes act as a barrier against invading pathogens and enteric infections are the consequences of multi-layered interactions among commensals, pathogens, and the host intestinal tissue. However, it remains unclear how perturbations of the gut microbiota compromise host infection resistance, especially through changes at species and metabolite levels. RESULTS: Here, we illustrate how Bacteroides vulgatus, a dominant species of the Bacteroidetes phylum in mouse intestine, suppresses infection by Vibrio cholerae, an important human pathogen. Clindamycin (CL) is an antibiotic that selectively kills anaerobic bacteria, and accordingly Bacteroidetes are completely eradicated from CL-treated mouse intestines. The Bacteroidetes-depleted adult mice developed severe cholera-like symptoms, when infected with V. cholerae. Germ-free mice mono-associated with B. vulgatus became resistant to V. cholerae infection. Levels of V. cholerae growth-inhibitory metabolites including short-chain fatty acids plummeted upon CL treatment, while levels of compounds that enhance V. cholerae proliferation were elevated. Furthermore, the intestinal colonization process of V. cholerae was well-simulated in CL-treated adult mice. CONCLUSIONS: Overall, we provide insights into how a symbiotic microbe and a pathogenic intruder interact inside host intestine. We identified B. vulgatus as an indigenous microbial species that can suppress intestinal infection. Our results also demonstrate that commensal-derived metabolites are a critical determinant for host resistance against V. cholerae infection, and that CL pretreatment of adult mice generates a simple yet useful model of cholera infection.
Files in This Item:
T201903491.pdf Download
DOI
10.1186/s40168-019-0746-y
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Nam, Ki Taek(남기택)
Ryu, Ji Hwan(유지환)
Yoon, Sang Sun(윤상선) ORCID logo https://orcid.org/0000-0003-2979-365X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/171418
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