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Establishment of PITX3-mCherry knock-in reporter human embryonic stem cell line (WAe009-A-23)

Authors
 Sanghyun Park  ;  Jeong-Eun Yoo  ;  Dongjin R. Lee  ;  Jiho Jang  ;  Myung Soo Cho  ;  Dae-Sung Kim  ;  Dong-Wook Kim 
Citation
 Stem Cell Research, Vol.39 : 101499, 2019 
Journal Title
 Stem Cell Research 
ISSN
 1873-5061 
Issue Date
2019
Abstract
Pituitary homeobox 3 (Pitx3) is a key transcription factor that plays an important role in the development and maintenance of midbrain dopaminergic (mDA) neurons. Here, we established a PITX3-mCherry knock-in reporter human embryonic stem cell (hESC) line using the CRISPR/Cas9 system. PITX3-mCherry hESCs maintained pluripotency marker expression and exhibited the capacity to generate all 3 germ layers and a normal karyotype. After differentiation into mDA neurons, most PITX3 immunoreactivity overlapped with the red fluorescence of mCherry. This reporter cell line may be used to study the development of mDA neurons or to enrich mDA populations for transplantation.
Files in This Item:
T201903220.pdf Download
DOI
10.1016/j.scr.2019.101499
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Kim, Dong Wook(김동욱) ORCID logo https://orcid.org/0000-0002-5025-1532
Yoo, Jeong Eun(유정은)
Jang, Ji Ho(장지호) ORCID logo https://orcid.org/0000-0001-5551-3514
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/171291
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