Cited 0 times in
Establishment of PITX3-mCherry knock-in reporter human embryonic stem cell line (WAe009-A-23)
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김동욱 | - |
dc.contributor.author | 장지호 | - |
dc.contributor.author | 유정은 | - |
dc.date.accessioned | 2019-10-28T01:40:25Z | - |
dc.date.available | 2019-10-28T01:40:25Z | - |
dc.date.issued | 2019 | - |
dc.identifier.issn | 1873-5061 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/171291 | - |
dc.description.abstract | Pituitary homeobox 3 (Pitx3) is a key transcription factor that plays an important role in the development and maintenance of midbrain dopaminergic (mDA) neurons. Here, we established a PITX3-mCherry knock-in reporter human embryonic stem cell (hESC) line using the CRISPR/Cas9 system. PITX3-mCherry hESCs maintained pluripotency marker expression and exhibited the capacity to generate all 3 germ layers and a normal karyotype. After differentiation into mDA neurons, most PITX3 immunoreactivity overlapped with the red fluorescence of mCherry. This reporter cell line may be used to study the development of mDA neurons or to enrich mDA populations for transplantation. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Elsevier | - |
dc.relation.isPartOf | Stem Cell Research | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Establishment of PITX3-mCherry knock-in reporter human embryonic stem cell line (WAe009-A-23) | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Physiology (생리학교실) | - |
dc.contributor.googleauthor | Sanghyun Park | - |
dc.contributor.googleauthor | Jeong-Eun Yoo | - |
dc.contributor.googleauthor | Dongjin R. Lee | - |
dc.contributor.googleauthor | Jiho Jang | - |
dc.contributor.googleauthor | Myung Soo Cho | - |
dc.contributor.googleauthor | Dae-Sung Kim | - |
dc.contributor.googleauthor | Dong-Wook Kim | - |
dc.identifier.doi | 10.1016/j.scr.2019.101499 | - |
dc.contributor.localId | A00406 | - |
dc.contributor.localId | A03480 | - |
dc.contributor.localId | A04775 | - |
dc.relation.journalcode | J02680 | - |
dc.identifier.eissn | 1876-7753 | - |
dc.identifier.pmid | 31352199 | - |
dc.contributor.alternativeName | Kim, Dong Wook | - |
dc.contributor.affiliatedAuthor | 김동욱 | - |
dc.contributor.affiliatedAuthor | 장지호 | - |
dc.contributor.affiliatedAuthor | 유정은 | - |
dc.citation.volume | 39 | - |
dc.citation.startPage | 101499 | - |
dc.identifier.bibliographicCitation | Stem Cell Research, Vol.39 : 101499, 2019 | - |
dc.identifier.rimsid | 63928 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.