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Optogenetic neuronal stimulation promotes axon outgrowth and myelination of motor neurons in a three-dimensional motor neuron-Schwann cell coculture model on a microfluidic biochip

Authors
 Sujin Hyung  ;  Seung‐Ryeol Lee  ;  Yeon Jee Kim  ;  Seokyoung Bang  ;  Dongha Tahk  ;  Jong‐Chul Park  ;  Jun‐Kyo Francis Suh  ;  Noo Li Jeon 
Citation
 Biotechnology and Bioengineering, Vol.116(10) : 2425-2438, 2019 
Journal Title
 Biotechnology and Bioengineering 
ISSN
 0006-3592 
Issue Date
2019
Keywords
Schwann cell (SC) ; motor neuron (MN) ; myelin ; optogenetically mediated light stimulation (OMLS) ; peripheral nerve trauma ; peripheral neuropathy
Abstract
Axonal regeneration and remyelination of peripheral motor neurons (MNs) are critical for restoring neuromuscular motor function after injury or peripheral neuropathy. We examined whether optogenetically mediated light stimulation (OMLS) could enhance the axon outgrowth and myelination of MNs using three-dimensional motor neuron-Schwann cell (MN-SC) coculture on a microfluidic biochip. The biochip was designed to allow SCs to interact with the axons of MNs, while preventing direct contact between SCs and the cell bodies of MNs. Following coculture with SCs on the microfluidic biochip, MNs were transfected with a light-sensitive channelrhodopsin gene. Transfected MNs subjected to repeated light stimulation (20 Hz, 1 hr) produced significantly longer axons than nontransfected MNs. OMLS of MNs greatly increased the number of myelin basic protein (MBP)-expressing SCs, promoting the initiation of myelination of MNs. Ultrastructurally, OMLS of MNs markedly enhanced the thickness of the compact myelin sheath around the MN axons such that the average thickness was closer to that of the theoretical estimates in vivo. Thus, the MN-SC coculture model on a microfluidic biochip augmented by OMLS of MNs is a feasible platform for studying the relationship of neuronal activity with regrowth and remyelination.
Full Text
https://onlinelibrary.wiley.com/doi/full/10.1002/bit.27083
DOI
10.1002/bit.27083
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/171243
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