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Experimental Tracheal Replacement Using 3-dimensional Bioprinted Artificial Trachea with Autologous Epithelial Cells and Chondrocytes

Authors
 Jae-Hyun Park  ;  Jeong-Kee Yoon  ;  Jung Bok Lee  ;  Young Min Shin  ;  Kang-Woog Lee  ;  Sang-Woo Bae  ;  JunHee Lee  ;  JunJie Yu  ;  Cho-Rok Jung  ;  Young-Nam Youn  ;  Hwi-Yool Kim  ;  Dae-Hyun Kim 
Citation
 Scientific Reports, Vol.9(1) : 2103, 2019 
Journal Title
 Scientific Reports 
Issue Date
2019
Abstract
Various treatment methods for tracheal defects have been attempted, such as artificial implants, allografts, autogenous grafts, and tissue engineering; however, no perfect method has been established. We attempted to create an effective artificial trachea via a tissue engineering method using 3D bio-printing. A multi-layered scaffold was fabricated using a 3D printer. Polycaprolactone (PCL) and hydrogel were used with nasal epithelial and auricular cartilage cells in the printing process. An artificial trachea was transplanted into 15 rabbits and a PCL scaffold without the addition of cells was transplanted into 6 rabbits (controls). All animals were followed up with radiography, CT, and endoscopy at 3, 6, and 12 months. In the control group, 3 out of 6 rabbits died from respiratory symptoms. Surviving rabbits in control group had narrowed tracheas due to the formation of granulation tissue and absence of epithelium regeneration. In the experimental group, 13 of 15 animals survived, and the histologic examination confirmed the regeneration of epithelial cells. Neonatal cartilage was also confirmed at 6 and 12 months. Our artificial trachea was effective in the regeneration of respiratory epithelium, but not in cartilage regeneration. Additional studies are needed to promote cartilage regeneration and improve implant stability.
Files in This Item:
T201902853.pdf Download
DOI
10.1038/s41598-019-38565-z
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Thoracic and Cardiovascular Surgery (흉부외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Dae-Hyun(김대현)
Youn, Young Nam(윤영남)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/171096
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