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Development of a HA1-specific enzyme-linked immunosorbent assay against pandemic influenza virus A H1N1

 Doo Hee Shim  ;  Min Jung Kim  ;  Hye-Ran Cha  ;  Eun Sun Park  ;  Ah Reum Kim  ;  Jeon Han Park  ;  Hyung-Cheon Park  ;  Daesub Song  ;  Jae Myun Lee 
 Clinical Experimental Vaccine Research, Vol.8(1) : 70-76, 2019 
Journal Title
 Clinical Experimental Vaccine Research 
Issue Date
Enzyme-linked immunosorbent assay ;  H1N1 subtype ; Infection ;  Influenza A virus ; Vaccination
PURPOSE: Enzyme-linked immunosorbent assay (ELISA) has been used in the diverse field to evaluate influenza virus infection; for the surveillance, diagnosis, efficacy evaluation, and development of the vaccine. The aim of this study was to establish an ELISA for detecting HA strain-specific antibodies using recombinant pandemic A H1N1 (pH1N1) HA1 (rHA1) protein. MATERIALS AND METHODS: rHA1 was produced in baculovirus system. The clinical performance of the developed ELISA was validated using human serum samples, by comparison with standard methods for detecting a neutralizing antibody; hemagglutination inhibition (HI) assay and microneutralization test (MNT). The ability of the ELISA system to evaluate the efficacy test of an influenza vaccine was explored by measuring antibody levels in the serum of vaccinated mice. RESULTS: Our ELISA could detect anti-rHA1 antibody in influenza-infected patients and vaccinated subjects. Compared to HI assay and MNT as reference methods, our method showed good performance in detection of anti-rHA1 antibody. Detection of the anti-rHA1 antibody in vaccinated mice and its correlation with titers in HI assay was also proved in a mice model. CONCLUSION: An ELISA system using rHA1 of pH1N1 influenza virus was developed, and showed good clinical performance in diagnosis of influenza virus infection and evaluation of the vaccination efficacy in both human and animal models.
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1. College of Medicine (의과대학) > Dept. of Pediatrics (소아청소년과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Min Jung(김민정) ORCID logo https://orcid.org/0000-0002-5634-9709
Park, Jeon Han(박전한) ORCID logo https://orcid.org/0000-0001-9604-3205
Park, Hyeong Cheon(박형천) ORCID logo https://orcid.org/0000-0002-1550-0812
Shim, Doo Hee(심두희) ORCID logo https://orcid.org/0000-0002-6696-7199
Lee, Jae Myun(이재면) ORCID logo https://orcid.org/0000-0002-5273-3113
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