0 79

Cited 2 times in

eIF4A3 Phosphorylation by CDKs Affects NMD during the Cell Cycle

Authors
 Incheol Ryu  ;  You-Sub Won  ;  Hongseok Ha  ;  Eunjin Kim  ;  Yeonkyoung Park  ;  Min Kyung Kim  ;  Do Hoon Kwon  ;  Junho Choe  ;  Hyun Kyu Song  ;  Hosung Jung  ;  Yoon Ki Kim 
Citation
 CELL REPORTS, Vol.26(8) : 2126-2139.e9, 2019 
Journal Title
 CELL REPORTS 
Issue Date
2019
Keywords
CDK ; cell cycle ; eIF4A3 ; exon junction complex ; nonsense-mediated mRNA decay ; phosphorylation
Abstract
Exon junction complexes (EJCs) loaded onto spliced mRNAs during splicing serve as molecular markers for various post-transcriptional gene-regulatory processes, including nonsense-mediated mRNA decay (NMD). Although the composition and structure of EJCs are well characterized, the mechanism regulating EJC deposition remains unknown. Here we find that threonine 163 (T163) within the RNA-binding motif of eIF4A3 (a core EJC component) is phosphorylated by cyclin-dependent protein kinases 1 and 2 in a cell cycle-dependent manner. T163 phosphorylation hinders binding of eIF4A3 to spliced mRNAs and other EJC components. Instead, it promotes association of eIF4A3 with CWC22, which guides eIF4A3 to an active spliceosome. These molecular events ensure the fidelity of specific deposition of the EJC ∼20-24 nt upstream of an exon-exon junction. Accordingly, NMD is affected by T163 phosphorylation. Collectively, our data provide evidence that T163 phosphorylation affects EJC formation and, consequently, NMD efficiency in a cell cycle-dependent manner.
Full Text
https://www.sciencedirect.com/science/article/pii/S221112471930138X
DOI
10.1016/j.celrep.2019.01.101
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Jung, Ho Sung(정호성) ORCID logo https://orcid.org/0000-0002-5059-8050
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/169897
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse