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DNA methylation of the ELOVL2, FHL2, KLF14, C1orf132/MIR29B2C, and TRIM59 genes for age prediction from blood, saliva, and buccal swab samples

Authors
 Sang-Eun Jung  ;  Seung Min Lim  ;  Sae Rom Hong  ;  Eun Hee Lee  ;  Kyoung-Jin Shin  ;  Hwan Young Lee 
Citation
 FORENSIC SCIENCE INTERNATIONAL-GENETICS, Vol.38 : 1-8, 2019 
Journal Title
 FORENSIC SCIENCE INTERNATIONAL-GENETICS 
ISSN
 1872-4973 
Issue Date
2019
MeSH
Acetyltransferases/genetics ; Adolescent ; Adult ; Aged ; Aging/genetics* ; Blood Chemical Analysis* ; CpG Islands/genetics ; DNA Methylation* ; Forensic Genetics ; Genetic Markers ; Genotyping Techniques/instrumentation ; Humans ; LIM-Homeodomain Proteins/genetics ; Membrane Proteins/genetics ; Metalloproteins/genetics ; Middle Aged ; Mouth Mucosa/chemistry* ; Muscle Proteins/genetics ; Saliva/chemistry* ; Sequence Analysis, DNA ; Sp Transcription Factors/genetics ; Transcription Factors/genetics ; Young Adult
Keywords
Age ; Blood ; Buccal swab ; DNA methylation ; Methylation SNaPshot ; Saliva
Abstract
Many studies have reported age-associated DNA methylation changes and age-predictive models in various tissues and body fluids. Although age-associated DNA methylation changes can be tissue-specific, a multi-tissue age predictor that is applicable to various tissues and body fluids with considerable prediction accuracy might be valuable. In this study, DNA methylation at 5 CpG sites from the ELOVL2, FHL2, KLF14, C1orf132/MIR29B2C, and TRIM59 genes were investigated in 448 samples from blood, saliva, and buccal swabs. A multiplex methylation SNaPshot assay was developed to measure DNA methylation simultaneously at the 5 CpG sites. Among the 5 CpG sites, 3 CpG sites in the ELOVL2, KLF14 and TRIM59 genes demonstrated strong correlation between DNA methylation and age in all 3 sample types. Age prediction models built separately for each sample type using the DNA methylation values at the 5 CpG sites showed high prediction accuracy with a Mean Absolute Deviation from the chronological age (MAD) of 3.478 years in blood, 3.552 years in saliva and 4.293 years in buccal swab samples. A tissue-combined model constructed with 300 training samples including 100 samples from each blood, saliva and buccal swab samples demonstrated a very strong correlation between predicted and chronological ages (r = 0.937) and a high prediction accuracy with a MAD of 3.844 years in the 148 independent test set samples of 50 blood, 50 saliva and 48 buccal swab samples. Although more validation might be needed, the tissue-combined model's prediction accuracies in each sample type were very much similar to those obtained from each tissue-specific model. The multiplex methylation SNaPshot assay and the age prediction models in our study would be useful in forensic analysis, which frequently involves DNA from blood, saliva, and buccal swab samples.
Full Text
https://www.sciencedirect.com/science/article/pii/S1872497318302382
DOI
10.1016/j.fsigen.2018.09.010
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Forensic Medicine (법의학과) > 1. Journal Papers
Yonsei Authors
Shin, Kyoung Jin(신경진) ORCID logo https://orcid.org/0000-0002-1059-9665
Jung, Sang Eun(정상은)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/169896
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