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Anti‐inflammatory effect of platelet‐rich plasma on nucleus pulposus cells with response of TNF‐α and IL‐1

 Ho‐Joong Kim  ;  Jin S. Yeom  ;  Yong‐Gon Koh  ;  Jee‐Eun Yeo  ;  Kyoung‐Tak Kang  ;  Young‐Mi Kang  ;  Bong‐Soon Chang  ;  Choon‐Ki Lee 
 JOURNAL OF ORTHOPAEDIC RESEARCH, Vol.32(4) : 551-556, 2014 
Journal Title
Issue Date
Collagen Type II/antagonists & inhibitors ; Collagen Type II/biosynthesis ; Cyclooxygenase 2/biosynthesis ; Cyclooxygenase 2/metabolism ; Down-Regulation/physiology ; Healthy Volunteers ; Humans ; Interleukin-1beta/physiology* ; Intervertebral Disc Degeneration/metabolism* ; Intervertebral Disc Degeneration/pathology ; Intervertebral Disc Degeneration/therapy* ; Matrix Metalloproteinase 3/biosynthesis ; Matrix Metalloproteinase 3/metabolism ; Middle Aged ; Platelet-Rich Plasma*/chemistry ; Platelet-Rich Plasma*/cytology ; Tumor Necrosis Factor-alpha/physiology
interleukin-1 ; nucleus pulposus cell ; platelet-rich plasma ; tumor necrosis factor-α
The purpose of this study was to investigate the anti-inflammatory effect of platelet-rich plasma (PRP) with collagen matrix on human nucleus pulposus (NP) cell in response to pro-inflammatory cytokines such as tumor necrosis factor-alpha (TNF-α) and interleukin-1 (IL-1). NP cells from human disks were cultured in a monolayer and maintained in the collagen matrix prior to the addition of recombinant human IL-1 and TNF-α. After applying IL-1 and TNF-α, PRP prepared by using a commercially available platelet concentration system was added. The response was investigated using real-time PCR for mRNA expression of type II collagen, aggrecan, matrix metalloproteinase-3 (MMP-3), and cyclooxygenase-2 (COX-2). The combination of IL-1β and TNF-α led to decrease of matrix synthesis gene expression such as collagen type II and aggrecan and increase of the degradation gene expression of COX-2 and MMP-3, compared to the control. Consecutive PRP exposure significantly recovered the down-regulated gene expression of collagen type II and aggrecan and significantly reduced the increased MMP-3 and COX-2 gene expression, compared to that of control groups with pro-inflammatory cytokines. The administration of PRP with collagen matrix markedly suppressed cytokine-induced pro-inflammatory degrading enzymes and mediators in the NP cell. It also rescued gene expression concerning matrix synthesis, thereby stabilizing NP cell differentiation.
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1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kang, Young Mi(강영미) ORCID logo https://orcid.org/0000-0002-7686-6316
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