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Forensic SNP Genotyping with SNaPshot: Development of a Novel In-house SBE Multiplex SNP Assay

Authors
 Mian Sahib Zar  ;  Ahmad Ali Shahid  ;  Muhammad Saqib Shahzad  ;  Kyoung‐Jin Shin  ;  Hwan Young Lee  ;  Sang‐Seob Lee  ;  Muhammad Israr  ;  Peter Wiegand  ;  Galina Kulstein 
Citation
 JOURNAL OF FORENSIC SCIENCES, Vol.63(6) : 1824-1829, 2018 
Journal Title
JOURNAL OF FORENSIC SCIENCES
ISSN
 0022-1198 
Issue Date
2018
Keywords
forensic genetics ; forensic genotyping ; forensic science ; multiplex development ; population genetics ; single nucleotide polymorphism ; single-base extension ; skeletal remains
Abstract
This study introduces a newly developed in-house SNaPshot single-base extension (SBE) multiplex assay for forensic single nucleotide polymorphism (SNP) genotyping of fresh and degraded samples. The assay was validated with fresh blood samples from four different populations. In addition, altogether 24 samples from skeletal remains were analyzed with the multiplex. Full SNP profiles could be obtained from 14 specimens, while ten remains showed partial SNP profiles. Minor allele frequencies (MAF) of bone samples and different populations were compared and used for association of skeletal remains with a certain population. The results reveal that the SNPs of the bone samples are genetically close to the Pathan population. The findings show that the new multiplex system can be utilized for SNP genotyping of degraded and forensic relevant skeletal material, enabling to provide additional investigative leads in criminal cases.
Full Text
https://onlinelibrary.wiley.com/doi/full/10.1111/1556-4029.13783
DOI
10.1111/1556-4029.13783
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Forensic Medicine (법의학과) > 1. Journal Papers
Yonsei Authors
Shin, Kyoung Jin(신경진) ORCID logo https://orcid.org/0000-0002-1059-9665
Lee, Hwan Young(이환영)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/167089
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