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Diagnostic Performance and Comparative Evaluation of the Architect, Liaison, and Platelia Epstein-Barr Virus Antibody Assays

 Younhee Park  ;  Borae G. Park  ;  Jihye Ha  ;  Hyon-Suk Kim 
 Annals of Laboratory Medicine, Vol.38(5) : 458-465, 2018 
Journal Title
 Annals of Laboratory Medicine 
Issue Date
Adolescent ; Adult ; Aged ; Aged, 80 and over ; Antibodies, Viral/analysis* ; Child ; Child, Preschool ; DNA, Viral/analysis ; Epstein-Barr Virus Infections/diagnosis* ; Epstein-Barr Virus Infections/virology ; Female ; Herpesvirus 4, Human/genetics ; Herpesvirus 4, Human/immunology* ; Herpesvirus 4, Human/isolation & purification ; Humans ; Immunoglobulin G/analysis ; Immunoglobulin M/analysis ; Infant ; Male ; Middle Aged ; Reagent Kits, Diagnostic ; Sensitivity and Specificity ; Young Adult
Antibody ; Assay ; DNA ; Diagnostic performance ; Epstein-Barr virus ; Immunoglobulin
BACKGROUND: Epstein-Barr Virus (EBV) is one of the most prevalent causes of viral infection in humans. EBV infection stage (acute, past, or absent infection) is typically determined using a combination of assays that detect EBV-specific markers, such as IgG and IgM antibodies against the EBV viral capsid antigen (VCA) and IgG antibodies against the EBV nuclear antigen (EBNA). We compared the diagnostic performance and agreement of results between three commercial EBV antibody assays using an EBV performance panel (SeraCare Life Science, Milford, MA, USA) as a reference. METHODS: EBV antibody tests of EBV VCA IgM, VCA IgG, and EBNA IgG antibodies were performed by the Architect (Abbott Diagnostics, Wiesbaden, Germany), Liaison (DiaSorin, Saluggia, Italy), and Platelia (Bio-Rad, Marnes-la-Coquette, France) assays. Agreement between the three assays was evaluated using 279 clinical samples, and EBV DNA and antibody test results were compared. RESULTS: The three EBV antibody assays showed good diagnostic performance with good and excellent agreement with the performance panel (kappa coefficient, >0.6). The overall VCA IgM positivity rate was higher in EBV DNA-positive samples than in EBV DNA-negative samples for all three EBV antibody assays (P=0.02). The three EBV antibody assays exhibited good agreement in results for the clinical samples. CONCLUSIONS: The diagnostic performance of the three EBV antibody assays was acceptable, and they showed comparable agreement in results for the clinical samples.
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1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
김현숙(Kim, Hyon Suk) ORCID logo https://orcid.org/0000-0001-5662-7740
박윤희(Park, Youn Hee) ORCID logo https://orcid.org/0000-0001-8458-1495
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