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Protein-Engineered Large Area Adipose-derived Stem Cell Sheets for Wound Healing

Authors
 Jongbeom Na  ;  Seung Yong Song  ;  Jae Dong Kim  ;  Minsu Han  ;  June Seok Heo  ;  Chae Eun Yang  ;  Hyun Ok Kim  ;  Dae Hyun Lew  ;  Eunkyoung Kim 
Citation
 Scientific Reports, Vol.8(1) : 15869, 2018 
Journal Title
 Scientific Reports 
Issue Date
2018
Abstract
Human adipose-derived stem cells (hADSCs) formed robust cell sheets by engineering the cells with soluble cell adhesive molecules (CAMs), which enabled unique approaches to harvest large area hADSC sheets. As a soluble CAM, fibronectin (FN) (100 pg/ml) enhanced the cell proliferation rate and control both cell-to-cell and cell-to-substrate interactions. Through this engineering of FN, a transferrable hADSC sheet was obtained as a free-stranding sheet (122.6 mm2) by a photothermal method. During the harvesting of hADSC sheets by the photothermal method, a collagen layer in-between cells and conductive polymer film (CP) was dissociated, to protect cells from direct exposure to a near infrared (NIR) source. The hADSC sheets were applied to chronic wound of genetically diabetic db/db mice in vivo, to accelerate 30% faster wound closure with a high closure effect (εwc) than that of control groups. These results indicated that the engineering of CAM and collagens allow hADSC sheet harvesting, which could be extended to engineer various stem cell sheets for efficient therapies.
Files in This Item:
T201804811.pdf.pdf Download
DOI
10.1038/s41598-018-34119-x
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Plastic and Reconstructive Surgery (성형외과학교실) > 1. Journal Papers
Yonsei Authors
김현옥(Kim, Hyun Ok) ORCID logo https://orcid.org/0000-0002-4964-1963
송승용(Song, Seung Yong) ORCID logo https://orcid.org/0000-0002-3145-7463
양채은(Yang, Chae Eun) ORCID logo https://orcid.org/0000-0001-8128-791X
유대현(Lew, Dae Hyun)
허준석(Heo, June Seok)
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/166826
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