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Optimizing tissue-clearing conditions based on analysis of the critical factors affecting tissue-clearing procedures

Authors
 June Hoan Kim  ;  Min Jee Jang  ;  Jungyoon Choi  ;  Eunsoo Lee  ;  Kyung–Deok Song  ;  Jaeho Cho  ;  Keun-Tae Kim  ;  Hyuk-Jin Cha  ;  Woong Sun 
Citation
 SCIENTIFIC REPORTS, Vol.8(1) : 12815, 2018 
Journal Title
 SCIENTIFIC REPORTS 
Issue Date
2018
Abstract
Tissue-clearing techniques have received great attention for volume imaging and for the potential to be applied in optical diagnosis. In principle, tissue clearing is achieved by reducing light scattering through a combination of lipid removal, size change, and matching of the refractive index (RI) between the imaging solution and the tissue. However, the contributions of these major factors in tissue clearing have not been systematically evaluated yet. In this study, we experimentally measured and mathematically calculated the contribution of these factors to the clearing of four organs (brain, liver, kidney, and lung). We found that these factors differentially influence the maximal clearing efficacy of tissues and the diffusivity of materials inside the tissue. We propose that these physical properties of organs can be utilized for the quality control (Q/C) process during tissue clearing, as well as for the monitoring of the pathological changes of tissues.
Files in This Item:
T201803211.pdf Download
DOI
10.1038/s41598-018-31153-7
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실) > 1. Journal Papers
Yonsei Authors
Cho, Jae Ho(조재호) ORCID logo https://orcid.org/0000-0001-9966-5157
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/166607
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