Change of angiogenesis-related protein expression by polydeoxyribonucleotide(PDRN) treatment in chondrocyte-like cells

Abstract

The purpose of this study was to investigate the effect of polydeoxynucleotide (PDRN) on factors associated with angiogenesis after administration of PDRN in chondrocyte-like cells. Interleukin (IL)-1β or phosphate buffered saline (PBS) was used to treat human chondrocytic cell line in hypoxic condition for 24 h (IL-1β group or control group). PDRN was then used to treat IL-1β group cells for 24 h (PDRN group). Angiopoietin-2 (ANG-2), platelet-derived growth factor (PDGF) related to pro-angiogenesis and angiostatin and endostatin related to anti-angiogenesis were chosen by Label-based Human Antibody Array 1000 for further validation studies. Quantitative real-time reverse transcription polymerase chain reaction and western blot analysis validated that levels of PDGF and ANG-2 were significantly increased in the PDRN group compared to those in the control group or the IL-1β group. However, levels of endostatin and angiostatin were significantly decreased in the PDRN group compared to those in the control group or the IL-1β group. In in vitro scratch assay, wound closure was significantly increased in the PDRN group compared to that in the control group or the IL-1β group. Moreover, PDRN decreased expression of MMP13 (a catabolic factor for OA) but increased expression of aggrecan (an anabolic factor for OA). These data suggest that PDRN may promote angiogenesis and wound healing via down-regulation of catabolism and up-regulation of anabolism in chondrocyte-like cells.