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TLR2, but not TLR4, plays a predominant role in the immune responses to cholera vaccines

 Jae Seung Yang  ;  Hye Jin Kim  ;  Seok‐Seong Kang  ;  Kyoung Whun Kim  ;  Dong Wook Kim  ;  Cheol‐Heui Yun  ;  Soon‐Jung Park  ;  Ho Seong Seo  ;  B. Brett Finlay  ;  Seung Hyun Han 
 Journal of Leukocyte Biology, Vol.98(4) : 661-669, 2015 
Journal Title
 Journal of Leukocyte Biology 
Issue Date
OmpU ; Vibrio cholerae ; innate immunity
Vibrio cholerae can cause severe diarrhea and dehydration leading to high mortality and morbidity. Current cholera vaccines are formulated with KVC. Although the innate immune responses following vaccination deeply influence the induction of adaptive immunity, the initial recognition of cholera vaccines by the host innate immune system is not well characterized. In this study, the ability of KVC to induce innate immune responses was investigated. Unlike typical Gram-negative bacteria stimulating TLR2 and TLR4, KVC activated TLR2 but hardly TLR4. However, purified V. cholerae LPS preferentially stimulated TLR4, although not as potently as LPS of other Gram-negative bacteria, implying that LPS is not a major immunostimulatory component of KVC. Instead, MPFs were similar to KVC in the capacity to activate TLR2, transcription factors, and cytokine expression. Furthermore, OmpU is an abundant membrane protein of V. cholerae and could interact with TLR2 for inducing cytokine expression. Notably, cholera vaccine-induced immune responses are impaired in TLR2(-/-) mice. Conclusively, TLR2 is essential for the immune responses to cholera vaccination, and OmpU is the major immunostimulatory component of cholera vaccines.
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1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Park, Soon Jung(박순정) ORCID logo https://orcid.org/0000-0002-0423-1944
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