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In Vivo Study of Natural Killer (NK) Cell Cytotoxicity Against Cholangiocarcinoma in a Nude Mouse Model.

Authors
 IN HYE JUNG  ;  DO HEE KIM  ;  DA KYUNG YOO  ;  SUN YOUNG BAEK  ;  SEONG HOON JEONG  ;  DAWOON E. JUNG  ;  SEUNG WOO PARK  ;  YONG-YOON CHUNG 
Citation
 IN VIVO, Vol.32(4) : 771-781, 2018 
Journal Title
 IN VIVO 
ISSN
 0258-851X 
Issue Date
2018
MeSH
Animals ; Cell Line, Tumor ; Cell Proliferation/genetics ; Cholangiocarcinoma/genetics ; Cholangiocarcinoma/immunology* ; Cholangiocarcinoma/pathology ; Cholangiocarcinoma/therapy ; Cytotoxicity, Immunologic/genetics ; Cytotoxicity, Immunologic/immunology* ; Disease Models, Animal ; Humans ; Immunotherapy, Adoptive/adverse effects* ; Killer Cells, Natural/immunology* ; Mice ; Mice, Nude ; Xenograft Model Antitumor Assays
Keywords
Cholangiocarcinoma ; NK cell ; xenograft nude mouse model
Abstract
BACKGROUND/AIM: Natural killer (NK) cells are one of the lymphocytes clinically used for various cancer types. Cytotoxicity of NK cells to cholangiocarcinoma (CC), however, has not yet been studied. Nor NK cell therapy against CC has been clinically applied. In this study, relevance of NK cell therapy for anti-tumor efficacy against CC was pre-clinically investigated. MATERIALS AND METHODS: Human HuCCT-1 cells, an intrahepatic CC cell line, were xenografted into nude mice. The HuCCT-1 tumor-bearing nude mice then received multiple infusions of ex vivo-expanded human NK cells (SMT01) and in vivo cytotoxic activity of the NK cells against the CC cells was evaluated. RESULTS: SMT01 infusion resulted in significant inhibition of the CC tumor growth. Body weight of the mice administrated with chemotherapy was found to be maintained at the lowest level among all treatment groups while all the SMT01 infusion groups well maintained their body weight. CONCLUSION: The present in vivo study demonstrates that NK cells contain cytolytic activity against cholangiocarcinoma and show beneficial effect of NK cell therapy in relevance to quality of life. Further investigation of the NK cell-based immunotherapy can be useful to determine cancer therapeutics for the specific tumor.
Files in This Item:
T201803887.pdf Download
DOI
10.21873/invivo.11307
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Park, Seung Woo(박승우) ORCID logo https://orcid.org/0000-0001-8230-964X
Jung, In Hye(정인혜) ORCID logo https://orcid.org/0000-0003-0553-0310
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/165354
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