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Fluorescence change of Fusobacterium nucleatum due to Porphyromonas gingivalis

Authors
 Min-Ah Lee  ;  Si-Mook Kang  ;  Se-Yeon Kim  ;  Ji-Soo Kim  ;  Jin-Bom Kim  ;  Seung-Hwa Jeong 
Citation
 Journal of Microbiology, Vol.56(9) : 628-633, 2018 
Journal Title
 Journal of Microbiology 
ISSN
 1225-8873 
Issue Date
2018
Keywords
Fusobacterium nucleatum ; Porphyromonas gingivalis ; QLF-D ; porphyrin ; red fluorescence
Abstract
The aim of this study was to measure changes in the fluorescence of Fusobacterium nucleatum interacting with Porphyromonas gingivalis for excitation with blue light at 405-nm. P. gingivalis was mono- and co-cultivated in close proximity with F. nucleatum. The fluorescence of the bacterial colonies was photographed using a QLF-D (Quantitative Light-induced Fluorescence-Digital) Biluminator camera system with a 405 nm light source and a specific filter. The red, green and blue intensities of fluorescence images were analyzed using the image analysis software. A fluorescence spectrometer was used to detect porphyrin synthesized by each bacterium. F. nucleatum, which emitted green fluorescence in single cultures, showed intense red fluorescence when it was grown in close proximity with P. gingivalis. F. nucleatum co-cultivated with P. gingivalis showed the same pattern of fluorescence peaks as for protoporphyrin IX in the red part of the spectrum. We conclude that the green fluorescence of F. nucleatum can change to red fluorescence in the presence of adjacent co-cultured with P. gingivalis, indicating that the fluorescence character of each bacterium might depend on the presence of other bacteria.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/163507
DOI
10.1007/s12275-018-7515-7
Appears in Collections:
1. Journal Papers (연구논문) > 2. College of Dentistry (치과대학) > Dept. of Preventive Dentistry and Public Oral Health (예방치과학교실)
Yonsei Authors
강시묵(Kang, Si-Mook) ORCID logo https://orcid.org/0000-0003-2341-3636
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