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Fluorescence change of Fusobacterium nucleatum due to Porphyromonas gingivalis

DC Field Value Language
dc.contributor.author강시묵-
dc.date.accessioned2018-10-11T08:57:43Z-
dc.date.available2018-10-11T08:57:43Z-
dc.date.issued2018-
dc.identifier.issn1225-8873-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/163507-
dc.description.abstractThe aim of this study was to measure changes in the fluorescence of Fusobacterium nucleatum interacting with Porphyromonas gingivalis for excitation with blue light at 405-nm. P. gingivalis was mono- and co-cultivated in close proximity with F. nucleatum. The fluorescence of the bacterial colonies was photographed using a QLF-D (Quantitative Light-induced Fluorescence-Digital) Biluminator camera system with a 405 nm light source and a specific filter. The red, green and blue intensities of fluorescence images were analyzed using the image analysis software. A fluorescence spectrometer was used to detect porphyrin synthesized by each bacterium. F. nucleatum, which emitted green fluorescence in single cultures, showed intense red fluorescence when it was grown in close proximity with P. gingivalis. F. nucleatum co-cultivated with P. gingivalis showed the same pattern of fluorescence peaks as for protoporphyrin IX in the red part of the spectrum. We conclude that the green fluorescence of F. nucleatum can change to red fluorescence in the presence of adjacent co-cultured with P. gingivalis, indicating that the fluorescence character of each bacterium might depend on the presence of other bacteria.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherMicrobiological Society of Korea-
dc.relation.isPartOfJOURNAL OF MICROBIOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleFluorescence change of Fusobacterium nucleatum due to Porphyromonas gingivalis-
dc.typeArticle-
dc.contributor.collegeCollege of Dentistry-
dc.contributor.departmentDept. of Preventive Dentistry-
dc.contributor.googleauthorMin-Ah Lee-
dc.contributor.googleauthorSi-Mook Kang-
dc.contributor.googleauthorSe-Yeon Kim-
dc.contributor.googleauthorJi-Soo Kim-
dc.contributor.googleauthorJin-Bom Kim-
dc.contributor.googleauthorSeung-Hwa Jeong-
dc.identifier.doi10.1007/s12275-018-7515-7-
dc.contributor.localIdA00049-
dc.relation.journalcodeJ01593-
dc.identifier.eissn1976-3794-
dc.identifier.pmid30141157-
dc.identifier.urlhttps://link.springer.com/article/10.1007/s12275-018-7515-7-
dc.subject.keywordFusobacterium nucleatum-
dc.subject.keywordPorphyromonas gingivalis-
dc.subject.keywordQLF-D-
dc.subject.keywordporphyrin-
dc.subject.keywordred fluorescence-
dc.contributor.alternativeNameKang, Si Mook-
dc.contributor.affiliatedAuthorKang, Si Mook-
dc.citation.volume56-
dc.citation.number9-
dc.citation.startPage628-
dc.citation.endPage633-
dc.identifier.bibliographicCitationJOURNAL OF MICROBIOLOGY, Vol.56(9) : 628-633, 2018-
dc.identifier.rimsid60455-
dc.type.rimsART-
Appears in Collections:
2. College of Dentistry (치과대학) > Others (기타) > 1. Journal Papers

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