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Platycarya strobilacea leaf extract inhibits tumor necrosis factor-α production and bone loss induced by Porphyromonas gingivalis-derived lipopolysaccharide

Authors
 Joo-Hee Lee  ;  Hyungkeun Kim  ;  Jae Hoon Shim  ;  Junhee Park  ;  Sun Kyoung Lee  ;  Kwang-Kyun Park  ;  Won-Yoon Chung 
Citation
 Archives of Oral Biology, Vol.96 : 46-51, 2018 
Journal Title
 Archives of Oral Biology 
ISSN
 0003-9969 
Issue Date
2018
Keywords
Bone resorption ; LPS ; Osteogenic activity ; Platycarya strobilacea extract ; Porphyromonas gingivalis ; TNF-α
Abstract
OBJECTIVE: Remodeling of alveolar bone is controlled by osteoclast-mediated bone resorption and osteoblast-induced bone formation. LPS of Porphyromonas gingivalis, a major causative agent of periodontitis, produces proinflammatory cytokines in host immune cells, which thereby triggers osteoclastogenesis and leads to alveolar bone resorption. We investigated the anti-periodontitis potential of Platycarya strobilacea leaf extract (PLE), which is used as a traditional medicine in Asian countries. DESIGN: TNF-α levels in cell culture media were measured using a commercially available enzyme-linked immunosorbent assay kit. Osteoclast differentiation was observed by tartrate-resistant acid phosphatase staining, and the expression levels of osteoclastogenic genes were measured by quantitative real-time PCR. Bone-resorbing activity was confirmed by the resorption pit formation, gelatin zymographic, and the cathepsin K activity assays. Osteogenic differentiation was confirmed with an ALP activity assay and alizarin red S staining. RESULTS: PLE treatment inhibited the production of TNF-α in P. gingivalis LPS-stimulated RAW264.7 macrophages. In bone marrow-derived macrophages serving as osteoclast precursors, PLE treatment blocked RANKL-induced osteoclastogenesis and gene expression levels of the osteoclastogenic transcription factor NFATc1, DC-STAMP for osteoclast fusion, and cathepsin K for osteoclast activity. In addition, PLE treatment reduced the formation of resorption pits and the secretion of MMP 9 and cathepsin K from the differentiated osteoclasts. Furthermore, PLE treatment induced osteogenesis by increasing ALP activity and calcium content in preosteoblastic cells. CONCLUSION: PLE inhibits P. gingivalis LPS-induced TNF-α production and bone resorption and induces bone formation. PLE may be a beneficial agent to promote oral health by inhibiting periodontitis-induced alveolar bone loss.
Full Text
https://www.sciencedirect.com/science/article/pii/S0003996918303406
DOI
10.1016/j.archoralbio.2018.08.011
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
박광균(Park, Kwang Kyun)
이선경(Lee, Sun Kyoung) ORCID logo https://orcid.org/0000-0002-3707-8050
이주희(Lee, Joo-Hee)
정원윤(Chung, Won Yoon) ORCID logo https://orcid.org/0000-0001-8428-9005
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/163298
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