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Retinoic acid and CTCF play key roles in inducing the collinear expression of the Hoxa cluster

 Ji Hoon Oh  ;  Clara Yuri Kim  ;  Ji-Yeon Lee  ;  Myoung Hee Kim 
 Acta Biochimica et Biophysica Sinica, Vol.50(6) : 555-559, 2018 
Journal Title
 Acta Biochimica et Biophysica Sinica 
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During the development of an embryo, the initiation of the collinear expression of Hox genes is essential for the proper formation of the anteroposterior body axis. Retinoic acid (RA), a natural derivative of vitamin A, plays a role in vertebrate development by regulating Hox gene expression. CCCTC-binding factor (CTCF), an insulator protein that controls gene transcription, also regulates the expression of Hox genes by binding to the CTCF-binding sites (CBSs). It has been reported that upon RA signaling, retinoic acid response elements (RAREs) located in the Hox clusters become occupied. Interestingly, RAREs exist in close proximity with CBSs, and therefore when RA is bound, CTCF cannot bind. Without CTCF and its insulator activities, the repressive domain in the chromatin becomes open for gene transcription. Here, we examine the relationship between RA and CTCF during the RA-induced expression of the Hoxa cluster genes, using F9 murine embryonic teratocarcinoma cells as a model system. We treated F9 cells with RA for different time, confirmed the collinear expression of Hoxa genes, and validated CTCF-binding in F9 cells as well as in CTCF-overexpressing F9 cells, in the presence of RA. The present study suggests that RA and CTCF pose antagonistic effects on each other during vertebrate development to attain Hox gene collinearity.
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1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Kim, Myoung Hee(김명희) ORCID logo https://orcid.org/0000-0001-5652-1452
Oh, Ji Hoon(오지훈) ORCID logo https://orcid.org/0000-0001-6619-5515
Lee, Ji Yeon(이지연) ORCID logo https://orcid.org/0000-0002-0670-3095
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