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Effects of the Th2-dominant milieu on allergic responses in Der f 1-activated mouse basophils and mast cells

Authors
 Myung-Hee Yi  ;  Hyoung-Pyo Kim  ;  Kyoung Yong Jeong  ;  Ju Yeong Kim  ;  In-Yong Lee  ;  Tai-Soon Yong 
Citation
 Scientific Reports, Vol.8(1) : 7706, 2018 
Journal Title
 Scientific Reports 
Issue Date
2018
Abstract
Although basophils and mast cells share similar phenotypic and functional properties, little is known about the difference in the initial Th2 immune responses of these cells following exposure to proteolytic allergens. Here, we investigated the mechanisms of Th2-mediated immune responses in mouse bone marrow-derived basophils (BMBs) and mast cells (BMMCs) via stimulation with the cysteine protease allergen Der f 1. Our results showed that Th2 cytokines were induced from BMBs by active recombinant Der f 1 (rDer f 1 independently with Toll-like receptor (TLR) 2 and TLR4. Although both BMBs and BMMCs expressed protease-activated receptors on their surfaces, PAR expression following exposure to rDer f 1 was altered only in basophils. G protein-coupled receptors in basophils were found to be associated with interleukin (IL)-4 and IL-13 production from BMBs upon Der f 1 treatment. Secretion of Th2 cytokines from rDer f 1-treated basophils was mediated by G protein betagamma and phosphatidylinositol 3-kinase gamma through the extracellular signal-regulated kinase and c-Jun N-terminal kinase pathways. These findings provide insights into the roles of cysteine proteases in Th2 immune responses, such as allergic diseases, and improve our understanding of the mechanisms of Th2 cytokine production.
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DOI
10.1038/s41598-018-25741-w
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실)
1. Journal Papers (연구논문) > 5. Research Institutes (연구소) > Institute of Allergy (알레르기연구소)
Yonsei Authors
김주영(Kim, Ju Young) ORCID logo https://orcid.org/0000-0003-2456-6298
김형표(Kim, Hyoung Pyo) ORCID logo https://orcid.org/0000-0003-1441-8822
용태순(Yong, Tai Soon)
이명희(Yi, Myung Hee) ORCID logo https://orcid.org/0000-0001-9537-5726
이인용(Lee, In Yong)
정경용(Jeong, Kyoung Yong) ORCID logo https://orcid.org/0000-0001-9887-1426
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/162590
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