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Suppression of T24 human bladder cancer cells by ROS from locally delivered hematoporphyrin-containing polyurethane films

 Dohyun Kim  ;  Mi Hee Lee  ;  Min-Ah Koo  ;  Byeong-Ju Kwon  ;  Min Sung Kim  ;  Gyeung Mi Seon  ;  Seung Hee Hong  ;  Jong-Chul Park 
 Photochemical & Photobiological Sciences, Vol.17(6) : 763-772, 2018 
Journal Title
 Photochemical & Photobiological Sciences 
Issue Date
Antineoplastic Agents/chemistry/*pharmacology ; Apoptosis/drug effects ; Cell Cycle/drug effects ; Cell Proliferation/drug effects ; Cell Survival/drug effects ; Drug Dose-Response Relationship ; *Drug Delivery Systems ; Antitumor Drug Screening Assays ; Hematoporphyrins/chemistry/*pharmacology ; Humans ; Photochemotherapy ; Photosensitizing Agents/chemistry/*pharmacology ; Polyurethanes/chemistry/*pharmacology ; Reactive Oxygen Species/analysis/*metabolism ; Structure-Activity Relationship ; Cultured Tumor Cells ; Ultraviolet Rays ; Urinary Bladder Neoplasms/*drug therapy/metabolism/pathology
Systemic injection of a photosensitizer is a general method in photodynamic therapy, but it has complications due to the unintended systemic distribution and remnants of photosensitizers. This study focused on the possibility of suppressing luminal proliferative cells by excessive reactive oxygen species from locally delivered photosensitizer with biocompatible polyurethane, instead of the systemic injection method. We used human bladder cancer cells, hematoporphyrin as the photosensitizer, and polyurethane film as the photosensitizer-delivering container. The light source was a self-made LED (510 nm, 5 mW cm-2) system. The cancer cells were cultured on different doses of hematoporphyrin-containing polyurethane film and irradiated with LED for 15 minutes and 30 minutes each. After irradiating with LED and incubating for 24 hours, cell viability analysis, cell cycle analysis, apoptosis assay, intracellular and extracellular ROS generation study and western blot were performed. The cancer cell suppression effects of different concentrations of the locally delivered hematoporphyrin with PDT were compared. Apoptosis dominant cancer cell suppressions were shown to be hematoporphyrin dose-dependent. However, after irradiation, intracellular ROS amounts were similar in all the groups having different doses of hematoporphyrin, but these values were definitely higher than those in the control group. Excessive extracellular ROS from the intended, locally delivered photosensitizer for photodynamic treatment application had an inhibitory effect on luminal proliferative cancer cells. This method can be another possibility for PDT application on contactable or attachable lesions.
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1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
Koo, Min-Ah(구민아) ORCID logo https://orcid.org/0000-0002-8671-1131
Kwon, Byeong-Ju(권병주) ORCID logo https://orcid.org/0000-0001-9916-0546
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
Seon, Gyeung Mi(선경미) ORCID logo https://orcid.org/0000-0002-0766-1106
Lee, Mi Hee(이미희) ORCID logo https://orcid.org/0000-0002-9630-7044
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