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Terminal Uridylyltransferases Execute Programmed Clearance of Maternal Transcriptome in Vertebrate Embryos

Authors
 Hyeshik Chang  ;  Jinah Yeo  ;  Jeong-Gyun Kim  ;  Hyunjoon Kim  ;  Jaechul Lim  ;  Mihye Lee  ;  Hyun Ho Kim  ;  Jiyeon Ohk  ;  Hee-Yeon Jeon  ;  Hyunsook Lee  ;  Hosung Jung  ;  Kyu-Won Kim  ;  V Narry Kim 
Citation
 Molecular Cell, Vol.70(1) : 72-82.e7, 2018 
Journal Title
 Molecular Cell 
ISSN
 1097-2765 
Issue Date
2018
Keywords
RNA decay ; TAIL-seq ; TUT4 ; TUT7 ; U tail ; Zcchc11 ; Zcchc6 ; maternal-to-zygotic transition ; poly(A) tail ; uridylation
Abstract
During the maternal-to-zygotic transition (MZT), maternal RNAs are actively degraded and replaced by newly synthesized zygotic transcripts in a highly coordinated manner. However, it remains largely unknown how maternal mRNA decay is triggered in early vertebrate embryos. Here, through genome-wide profiling of RNA abundance and 3' modification, we show that uridylation is induced at the onset of maternal mRNA clearance. The temporal control of uridylation is conserved in vertebrates. When the homologs of terminal uridylyltransferases TUT4 and TUT7 (TUT4/7) are depleted in zebrafish and Xenopus, maternal mRNA clearance is significantly delayed, leading to developmental defects during gastrulation. Short-tailed mRNAs are selectively uridylated by TUT4/7, with the highly uridylated transcripts degraded faster during the MZT than those with unmodified poly(A) tails. Our study demonstrates that uridylation plays a crucial role in timely mRNA degradation, thereby allowing the progression of early development.
Full Text
https://www.sciencedirect.com/science/article/pii/S1097276518301850
DOI
10.1016/j.molcel.2018.03.004
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Jung, Ho Sung(정호성) ORCID logo https://orcid.org/0000-0002-5059-8050
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/162378
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