0 62

Cited 0 times in

Activated leucocyte cell adhesion molecule (ALCAM/CD166) regulates T cell responses in a murine model of food allergy

Authors
 Y S Kim  ;  M N Kim  ;  K E Lee  ;  J Y Hong  ;  M S Oh  ;  S Y Kim  ;  K W Kim  ;  M H Sohn 
Citation
 Clinical and Experimental Immunology, Vol.192(2) : 151-164, 2018 
Journal Title
 Clinical and Experimental Immunology 
ISSN
 0009-9104 
Issue Date
2018
Keywords
ALCAM ; CD166 ; activated leucocyte cell adhesion molecule ; food allergy
Abstract
Food allergy is a major public health problem. Studies have shown that long-term interactions between activated leucocyte cell adhesion molecule (ALCAM/CD166) on the surface of antigen-presenting cells, and CD6, a co-stimulatory molecule, influence immune responses. However, there are currently no studies on the functions of ALCAM in food allergy. Therefore, we aimed to identify the functions of ALCAM in ovalbumin (OVA)-induced food allergy using ALCAM-deficient mice. Wild-type (WT) and ALCAM-deficient (ALCAM(-/-) ) mice were sensitized intraperitoneally and with orally fed OVA. The mice were killed, and parameters related to food allergy and T helper type 2 (Th2) immune responses were analysed. ALCAM serum levels increased and mRNA expression decreased in OVA-challenged WT mice. Serum immunoglobulin (Ig)E levels, Th2 cytokine mRNA and histological injuries were higher in OVA-challenged WT mice than in control mice, and these were attenuated in ALCAM(-/-) mice. T cell proliferation of total cells, CD3(+) CD4(+) T cells and activated T cells in immune tissues were diminished in OVA-challenged ALCAM(-/-) mice. Proliferation of co-cultured T cells and dendritic cells (DCs) was decreased by the anti-CD6 antibody. In addition, WT mice sensitized by adoptive transfer of OVA-pulsed ALCAM(-/-) BM-derived DCs showed reduced immune responses. Lastly, serum ALCAM levels were higher in children with food allergy than in control subjects. In this study, serum levels of ALCAM were elevated in food allergy-induced WT mice and children with food allergy. Moreover, immune responses and T cell activation were attenuated in OVA-challenged ALCAM(-/-) mice. These results indicate that ALCAM regulates food allergy by affecting T cell activation.
Full Text
https://onlinelibrary.wiley.com/doi/abs/10.1111/cei.13104
DOI
10.1111/cei.13104
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Pediatrics (소아청소년과학교실)
1. Journal Papers (연구논문) > 2. College of Dentistry (치과대학) > Others (기타)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원)
Yonsei Authors
김경원(Kim, Kyung Won) ORCID logo https://orcid.org/0000-0003-4529-6135
김미나(Kim, Mina) ORCID logo https://orcid.org/0000-0002-1675-0688
김수연(Kim, Soo Yeon) ORCID logo https://orcid.org/0000-0003-4965-6193
손명현(Sohn, Myung Hyun) ORCID logo https://orcid.org/0000-0002-2478-487X
홍정연(Hong, Jung Yeon) ORCID logo https://orcid.org/0000-0003-0406-9956
Export
RIS (EndNote)
XLS (Excel)
XML
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/162316
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse