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Comparison between Newly Developed and Commercial Inhalant Skin Prick Test Reagents Using In Vivo and In Vitro Methods

Authors
 Sang Chul Lee  ;  Da Woon Sim  ;  Jongsun Lee  ;  Kyoung Yong Jeong  ;  Kyung Hee Park  ;  Jae Hyun Lee  ;  Jung Dong Kim  ;  Jung Won Park 
Citation
 Journal of Korean Medical Science, Vol.33(13) : e101, 2018 
Journal Title
 Journal of Korean Medical Science 
ISSN
 1011-8934 
Issue Date
2018
Keywords
Allergens ; Allergy and Immunology ; Diagnosis ; Skin Prick Test
Abstract
BACKGROUND: We developed skin prick test (SPT) reagents for common inhalant allergens that reflected the real exposure in Korea. The study aim was to evaluate diagnostic usefulness and allergen potency of our inhalant SPT reagents in comparison with commercial products. METHODS: We produced eight common inhalant allergen SPT reagents using total extract (Prolagen): Dermatophagoides farinae, Dermatophagoides pteronyssinus, oak, ragweed, mugwort, Humulus japonicus pollens, as well as cat and dog allergens. We compared the newly developed reagents with three commercially available SPT reagents (Allergopharma, Hollister-Stier, Lofarma). We measured total protein concentrations, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), major allergen concentration, and biological allergen potencies measured by immunoglobulin E (IgE) immunoblotting and ImmunoCAP inhibition test. RESULTS: Diagnostic values of these SPT reagents were expressed as positivity rate and concordance rate of the results from ImmunoCAP allergen-specific IgE test in 94 allergic patients. In vitro analysis showed marked differences in protein concentrations, SDS-PAGE features, major allergen concentrations, and biological allergen potencies of four different SPT reagents. In vivo analysis showed that positive rates and concordance rates of Prolagen(R) SPT reagents were similar compared to the three commercial SPT reagents. CONCLUSION: The newly developed Prolagen(R) inhalant SPT reagents are not inferior to the commercially available SPT reagents in allergy diagnosis.
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DOI
10.3346/jkms.2018.33.e101
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Institute of Allergy (알레르기연구소) > 1. Journal Papers
Yonsei Authors
Park, Kyung Hee(박경희) ORCID logo https://orcid.org/0000-0003-3605-5364
Park, Jung Won(박중원) ORCID logo https://orcid.org/0000-0003-0249-8749
Lee, Sang Chul(이상철)
Lee, Jae Hyun(이재현) ORCID logo https://orcid.org/0000-0002-0760-0071
Jeong, Kyoung Yong(정경용) ORCID logo https://orcid.org/0000-0001-9887-1426
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/162159
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