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Down-regulation of B-Cell Translocation Gene 1 by Promoter Methylation in Colorectal Carcinoma

Authors
 Yoon Yang Jung  ;  Ji-Youn Sung  ;  Ji-Ye Kim  ;  Hyun-Soo Kim 
Citation
 Anticancer Research, Vol.38(2) : 691-697, 2018 
Journal Title
 Anticancer Research 
ISSN
 0250-7005 
Issue Date
2018
MeSH
Tumor/genetics Biomarkers ; Case-Control Studies ; Tumor Cell Line ; Colorectal Neoplasms/*genetics/pathology ; *DNA Methylation ; Down-Regulation/genetics ; Neoplastic Gene Expression Regulation ; Tumor Suppressor Genes ; Humans ; Neoplasm Proteins/*genetics/metabolism ; Genetic *Promoter Regions
Keywords
B-cell translocation gene 1 ; Colon ; colorectal carcinoma ; down-regulation ; promoter methylation
Abstract
BACKGROUND: B-cell translocation gene 1 (BTG1) acts as a tumour suppressor in human malignancies. However, the precise mechanism of BTG1 down-regulation in colorectal carcinoma (CRC) remains unclear. We analyzed BTG1 expression in CRC cell lines and tissues and investigated the mechanism underlying the observed alterations. MATERIALS AND METHODS: Real-time polymerase chain reaction (PCR) and western blot analyses were performed to analyze BTG1 expression in CRC cell lines. The methylation status of the BTG1 promoter region in cell lines was determined by methylation-specific PCR, and the effect of demethylation on BTG1 expression was explored with 5-aza-deoxycytidine treatment. BTG1 protein expression in CRC tissue samples was evaluated using immunostaining. RESULTS: CRC cell lines and tissue samples expressed lower levels of BTG1 compared to controls, and BTG1 levels were significantly lower in metastatic than primary CRC. In BTG1-down-regulated CRC cell lines, the BTG1 promoter was highly methylated, and 5-aza-deoxycytidine significantly restored BTG1 expression. CONCLUSION: BTG1 down-regulation in CRC occurs through epigenetic repression, which is involved in the development and progression of CRC.
Full Text
http://ar.iiarjournals.org/content/38/2/691.abstract
DOI
10.21873/anticanres.12274
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyun-Soo(김현수)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/162060
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