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Down-regulation of B-Cell Translocation Gene 1 by Promoter Methylation in Colorectal Carcinoma

DC FieldValueLanguage
dc.contributor.author김현수-
dc.date.accessioned2018-08-28T16:54:52Z-
dc.date.available2018-08-28T16:54:52Z-
dc.date.issued2018-
dc.identifier.issn0250-7005-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/162060-
dc.description.abstractBACKGROUND: B-cell translocation gene 1 (BTG1) acts as a tumour suppressor in human malignancies. However, the precise mechanism of BTG1 down-regulation in colorectal carcinoma (CRC) remains unclear. We analyzed BTG1 expression in CRC cell lines and tissues and investigated the mechanism underlying the observed alterations. MATERIALS AND METHODS: Real-time polymerase chain reaction (PCR) and western blot analyses were performed to analyze BTG1 expression in CRC cell lines. The methylation status of the BTG1 promoter region in cell lines was determined by methylation-specific PCR, and the effect of demethylation on BTG1 expression was explored with 5-aza-deoxycytidine treatment. BTG1 protein expression in CRC tissue samples was evaluated using immunostaining. RESULTS: CRC cell lines and tissue samples expressed lower levels of BTG1 compared to controls, and BTG1 levels were significantly lower in metastatic than primary CRC. In BTG1-down-regulated CRC cell lines, the BTG1 promoter was highly methylated, and 5-aza-deoxycytidine significantly restored BTG1 expression. CONCLUSION: BTG1 down-regulation in CRC occurs through epigenetic repression, which is involved in the development and progression of CRC.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherInternational Institute of Anticancer Research-
dc.relation.isPartOfANTICANCER RESEARCH-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHTumor/genetics Biomarkers-
dc.subject.MESHCase-Control Studies-
dc.subject.MESHTumor Cell Line-
dc.subject.MESHColorectal Neoplasms/*genetics/pathology-
dc.subject.MESH*DNA Methylation-
dc.subject.MESHDown-Regulation/genetics-
dc.subject.MESHNeoplastic Gene Expression Regulation-
dc.subject.MESHTumor Suppressor Genes-
dc.subject.MESHHumans-
dc.subject.MESHNeoplasm Proteins/*genetics/metabolism-
dc.subject.MESHGenetic *Promoter Regions-
dc.titleDown-regulation of B-Cell Translocation Gene 1 by Promoter Methylation in Colorectal Carcinoma-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Pathology-
dc.contributor.googleauthorYoon Yang Jung-
dc.contributor.googleauthorJi-Youn Sung-
dc.contributor.googleauthorJi-Ye Kim-
dc.contributor.googleauthorHyun-Soo Kim-
dc.identifier.doi10.21873/anticanres.12274-
dc.contributor.localIdA01114-
dc.relation.journalcodeJ00188-
dc.identifier.eissn1791-7530-
dc.identifier.pmid29374692-
dc.identifier.urlhttp://ar.iiarjournals.org/content/38/2/691.abstract-
dc.subject.keywordB-cell translocation gene 1-
dc.subject.keywordColon-
dc.subject.keywordcolorectal carcinoma-
dc.subject.keyworddown-regulation-
dc.subject.keywordpromoter methylation-
dc.contributor.alternativeNameKim, Hyun-Soo-
dc.contributor.affiliatedAuthorKim, Hyun-Soo-
dc.citation.volume38-
dc.citation.number2-
dc.citation.startPage691-
dc.citation.endPage697-
dc.identifier.bibliographicCitationANTICANCER RESEARCH, Vol.38(2) : 691-697, 2018-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers

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