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Comparison of three rapid influenza diagnostic tests with digital readout systems and one conventional rapid influenza diagnostic test

Authors
 Sook Won Ryu  ;  In Bum Suh  ;  Se-Min Ryu  ;  Kyu Sung Shin  ;  Hyon-Suk Kim  ;  Juwon Kim  ;  Young Uh  ;  Kap Jun Yoon  ;  Jong-Han Lee 
Citation
 JOURNAL OF CLINICAL LABORATORY ANALYSIS, Vol.32(2) : e22234, 2018 
Journal Title
 JOURNAL OF CLINICAL LABORATORY ANALYSIS 
ISSN
 0887-8013 
Issue Date
2018
MeSH
Adolescent ; Adult ; Child ; Preschool Child ; Routine/*methods Diagnostic Tests ; Fluorescent Antibody Technique ; Humans ; Immunoassay/*methods ; Infant ; Newborn Infant ; Human/*diagnosis Influenza ; Nasopharynx/virology ; Sensitivity and Specificity ; Virology/*methods ; Young Adult
Keywords
digital readout system ; influenza ; rapid influenza diagnostic test ; sensitivity ; specificity
Abstract
BACKGROUND: Rapid influenza diagnostic tests (RIDTs) show variable sensitivities in clinical settings. We aimed to compare three digital RIDTs and one conventional RIDT. METHODS: We assessed 218 nasopharyngeal swabs from patients between neonates and 90 years old in 2016. Three digital RIDTs were BUDDI, Sofia Influenza A+B Fluorescence Immunoassay, Veritor System Flu A+B assay. One conventional test was the SD Bioline Influenza Ag A/B/A(H1N1/2009). All test results were compared with those from the Anyplex Flu A/B Typing Real-time Detection real-time PCR. The four RIDTs were tested with diluted solutions from the National Institute for Biological Standards and Control (NIBSC) to compare lower detection limit. Cross-reactivity of four RIDTs within other respiratory viruses was identified. RESULTS: For influenza A, BUDDI, Sofia, Veritor, and Bioline showed 87.7%, 94.5%, 87.7%, and 72.6% sensitivity, and 100%, 97.7%, 96.5%, and 100% specificity. For influenza B, BUDDI, Sofia, Veritor, and Bioline showed 81.7%, 91.7%, 81.7%, and 78.3% sensitivity, and 100%, 95.3%, 100%, and 100% specificity, respectively. Each RIDT could detect diluted NIBSC solution, according to the level of dilution and specific influenza subtypes. Cross-reactivity of four RIDTs with other respiratory viruses was not noted. CONCLUSIONS: Sofia showed the highest sensitivity for influenza A and B detection. BUDDI and Veritor showed higher detection sensitivity than a conventional RIDT for influenza A detection, but similar results for influenza B detection. Further study is needed to compare the test performance of RIDTs according to specific, prevalent influenza subtypes.
Full Text
https://onlinelibrary.wiley.com/doi/abs/10.1002/jcla.22234
DOI
10.1002/jcla.22234
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyon Suk(김현숙) ORCID logo https://orcid.org/0000-0001-5662-7740
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/161948
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