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Effects of structurally stabilized EGF and bFGF on wound healing in type I and type II diabetic mice

DC FieldValueLanguage
dc.contributor.author박광환-
dc.contributor.author이경미-
dc.contributor.author이진우-
dc.contributor.author백다운-
dc.date.accessioned2018-08-28T16:45:35Z-
dc.date.available2018-08-28T16:45:35Z-
dc.date.issued2018-
dc.identifier.issn1742-7061-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/161909-
dc.description.abstractDiabetes mellitus comprises a multiple metabolic disorder that affects millions of people worldwide and consequentially poses challenges for clinical treatment. Among the various complications, diabetic ulcer constitutes the most prevalent associated disorder and leads to delayed wound healing. To enhance wound healing capacity, we developed structurally stabilized epidermal growth factor (ST-EGF) and basic fibroblast growth factor (ST-bFGF) to overcome limitations of commercially available EGF (CA-EGF) and bFGF (CA-bFGF), such as short half-life and loss of activity after loading onto a matrix. Neither ST-EGF nor ST-bFGF was toxic, and both were more stable at higher temperatures than CA-EGF and CA-bFGF. We loaded ST-EGF and ST-bFGF onto a hyaluronate-collagen dressing (HCD) matrix, a biocompatible carrier, and tested the effectiveness of this system in promoting wound healing in a mouse model of diabetes. Wounds treated with HCD matrix loaded with 0.3mug/cm(2) ST-EGF or 1mug/cm(2) ST-bFGF showed a more rapid rate of tissue repair as compared to the control in type I and II diabetes models. Our results indicate that an HDC matrix loaded with 0.3mug/cm(2) ST-EGF or 1mug/cm(2) ST-bFGF can promote wound healing in diabetic ulcers and are suitable for use in wound dressings owing to their stability for long periods at room temperature. STATEMENT OF SIGNIFICANCE: Various types of dressing materials loaded with growth factors, such as VEGF, EGF, and bFGF, are widely used to effect wound repair. However, such growth factor-loaded materials have several limitations for use as therapeutic agents in healing-impaired diabetic wounds. To overcome these limitations, we have developed new materials containing structurally stabilized EGF (ST-EGF) and bFGF (ST-bFGF). To confirm the wound healing capacity of newly developed materials (ST-EGF and ST-bFGF-loaded hyaluronate-collagen dressing [HCD] matrix), we applied these matrices in type I and type II diabetic wounds. Notably, these matrices were able to accelerate wound healing including re-epithelialization, neovascularization, and collagen deposition. Consequentially, these ST-EGF and ST-bFGF-loaded HCD matrix may be used as future therapeutic agents in patients with diabetic foot ulcers.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherElsevier-
dc.relation.isPartOfACTA BIOMATERIALIA-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleEffects of structurally stabilized EGF and bFGF on wound healing in type I and type II diabetic mice-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Orthopedic Surgery-
dc.contributor.googleauthorSeong Mi Choi-
dc.contributor.googleauthorKyoung-Mi Lee-
dc.contributor.googleauthorHyun Jung Kim-
dc.contributor.googleauthorIk Kyu Park-
dc.contributor.googleauthorHwi Ju Kang-
dc.contributor.googleauthorHang-Cheol Shin-
dc.contributor.googleauthorDawoon Baek-
dc.contributor.googleauthorYoorim Choi-
dc.contributor.googleauthorKwang Hwan Park-
dc.contributor.googleauthorJin Woo Lee-
dc.identifier.doi10.1016/j.actbio.2017.11.045-
dc.contributor.localIdA01437-
dc.contributor.localIdA04619-
dc.contributor.localIdA03230-
dc.contributor.localIdA05642-
dc.relation.journalcodeJ00007-
dc.identifier.eissn1878-7568-
dc.identifier.pmid29203426-
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S1742706117307420-
dc.contributor.alternativeNamePark, Kwang Hwan-
dc.contributor.alternativeNameLee, Kyoung Mi-
dc.contributor.alternativeNameLee, Jin Woo-
dc.contributor.affiliatedAuthorPark, Kwang Hwan-
dc.contributor.affiliatedAuthorLee, Kyoung Mi-
dc.contributor.affiliatedAuthorLee, Jin Woo-
dc.citation.volume66-
dc.citation.startPage325-
dc.citation.endPage334-
dc.identifier.bibliographicCitationACTA BIOMATERIALIA, Vol.66 : 325-334, 2018-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers

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