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Comparative Genome Analysis and Global Phylogeny of the Toxin Variant Clostridium difficile PCR Ribotype 017 Reveals the Evolution of Two Independent Sublineages (vol 55, pg 865, 2017)

 M. D. Cairns  ;  M. D. Preston  ;  C. L. Hall  ;  D. N. Gerding  ;  P. M. Hawkey  ;  H. Kato  ;  H. Kim  ;  E. J. Kuijper  ;  T. D. Lawley  ;  H. Pituch  ;  S. Reid  ;  B. Kullin  ;  T. V. Riley  ;  K. Solomon  ;  P. J. Tsai  ;  J. S. Weese  ;  R. A. Stabler  ;  B. W. Wren 
 JOURNAL OF CLINICAL MICROBIOLOGY, Vol.55(3) : 865-876, 2017 
Journal Title
Issue Date
Animals ; Clostridium Infections/epidemiology ; Clostridium Infections/microbiology* ; Clostridium Infections/veterinary* ; Clostridium difficile/classification* ; Clostridium difficile/genetics* ; Clostridium difficile/isolation & purification ; Genetic Variation* ; Genome, Bacterial ; Global Health ; Humans ; Molecular Epidemiology ; Phylogeny* ; Polymerase Chain Reaction ; Ribotyping* ; Sequence Analysis, DNA
Clostridium difficile ; SNPs ; antibiotic resistance ; evolution ; phylogenetics ; phylogeny ; ribotype 017 ; sequencing
The diarrheal pathogen Clostridium difficile consists of at least six distinct evolutionary lineages. The RT017 lineage is anomalous, as strains only express toxin B, compared to strains from other lineages that produce toxins A and B and, occasionally, binary toxin. Historically, RT017 initially was reported in Asia but now has been reported worldwide. We used whole-genome sequencing and phylogenetic analysis to investigate the patterns of global spread and population structure of 277 RT017 isolates from animal and human origins from six continents, isolated between 1990 and 2013. We reveal two distinct evenly split sublineages (SL1 and SL2) of C. difficile RT017 that contain multiple independent clonal expansions. All 24 animal isolates were contained within SL1 along with human isolates, suggesting potential transmission between animals and humans. Genetic analyses revealed an overrepresentation of antibiotic resistance genes. Phylogeographic analyses show a North American origin for RT017, as has been found for the recently emerged epidemic RT027 lineage. Despite having only one toxin, RT017 strains have evolved in parallel from at least two independent sources and can readily transmit between continents.
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1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Heejung(김희정) ORCID logo https://orcid.org/0000-0002-0190-703X
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