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Ginsenoside Rg3 Decreases Fibrotic and Invasive Nature of Endometriosis by Modulating miRNA-27b: In Vitro and In Vivo Studies

Authors
 Min Kyoung Kim  ;  Seung Kyun Lee  ;  Ji Hyun Park  ;  Jae Hoon Lee  ;  Bo Hyon Yun  ;  Joo Hyun Park  ;  Seok Kyo Seo  ;  SiHyun Cho  ;  Young Sik Choi 
Citation
 Scientific Reports, Vol.7(1) : 17670, 2017 
Journal Title
 Scientific Reports 
Issue Date
2017
Abstract
This research aimed to evaluate the potential therapeutic effects of Rg3 on endometriosis and identify target miRNAs. We designed an in vitro study using human endometrial stromal cells (HESCs) obtained from patients with endometriosis and an in vivo study using mouse models. HESCs were treated with Rg3-enhanced red ginseng extract (Rg3E); real-time PCR and microarray profiling, transfection, and western blot were performed. Mouse endometriosis models were developed and supplemented with Rg3E for 8 weeks. Gross lesion size and fibrotic character were analyzed in the mouse models. RNA levels of Ki-67, col-1, CTGF, fibronectin, TGF-β1, MMP2 and MMP9 significantly decreased in HESCs after Rg3E treatment. Microarray analysis revealed downregulation of miR-27b-3p, which is related to fibrosis modulation. Expression of miR-27b-3p was significantly higher in HESCs from patients with endometriosis than that of controls, and Rg3E treatment significantly decreased its expression; the contraction and migration assay revealed significant reductions in both fibrosis and migration potential in Rg3E-treated HESCs from endometriosis patients. A decrease in size and fibrotic character of endometrial lesions from the Rg3E groups was observed in vivo. In conclusion, Rg3 effectively altered fibrotic properties of HESCs from patients with endometriosis, which is likely associated with miR-27b-3p modulation.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/161528
DOI
10.1038/s41598-017-17956-0
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실)
Yonsei Authors
김민경(Kim, Min Kyoung) ; 박주현(Park, Joo Hyun) ; 서석교(Seo, Seok Kyo) ; 윤보현(Yun, Bo Hyon) ; 이재훈(Lee, Jae Hoon) ; 조시현(Cho, Si Hyun) ; 최영식(Choi, Young Sik)
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