0 412

Cited 1 times in

Comparison of Abbott RealTime genotype II, GeneMatrix restriction fragment mass polymorphism and Sysmex HISCL HCV Gr assays for hepatitis C virus genotyping

Authors
 Mi-Soon Han  ;  Yongjung Park  ;  Hyon-Suk Kim 
Citation
 CLINICAL CHEMISTRY AND LABORATORY MEDICINE, Vol.55(8) : 1122-1128, 2017 
Journal Title
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
ISSN
 1434-6621 
Issue Date
2017
MeSH
Genotyping Techniques/methods* ; Hepacivirus/genetics* ; Humans ; Limit of Detection ; Sequence Analysis, DNA/methods* ; Time Factors
Keywords
Abbott RealTime ; HCV genotype ; Sysmex HISCL Gr ; nucleic acid amplification test (NAAT) ; restriction fragment mass polymorphism (RFMP) ; serology
Abstract
BACKGROUND: Hepatitis C virus (HCV) genotype is a predictive marker for treatment response. We sequentially evaluated the performances of two nucleic acid amplification tests (NAATs) and one serology assay for HCV genotype: Abbott RealTime genotype II (RealTime II), GeneMatrix restriction fragment mass polymorphism (RFMP), and Sysmex HISCL HCV Gr (HISCL Gr).

METHODS: We examined 281 clinical samples with three assays. The accuracy was assessed using the HCV Genotype Performance Panel PHW204 (SeraCare Life Sciences) for two NAATs. Discrepant cases were re-genotyped by the Versant HCV v.2.0 (line probe 2.0) assay.

RESULTS: With the RealTime II assay, clinic samples were analyzed as follows: genotypes 1b (43.1%), 2 (40.2%), 1 subtypes other than 1a and 1b (12.5%), 3 (1.8%), 4 (1.4%), 1a (0.7%), 6 (0.4%), and mixed (1.1%). The RealTime II and RFMP assays showed a type concordance rate of 97.5% (274/281) (κ=0.80) and no significant discordance (p=0.25). Both assays accurately genotyped all samples in the Performance Panel by the subtype level. The HISCL Gr assay showed concordance rates of about 91% (κ<0.40) and statistically significant discordances with two NAATs (p<0.05). In confirmation tests, the results of RFMP assay were the most consistent with those of Versant 2.0 assay.

CONCLUSIONS: The three HCV assays provided genotyping and serotyping results with good concordance rates. The two NAATs (RealTime II and RFMP) showed comparable performance and good agreement. However, the results of the HISCL Gr assay showed statistically significant differences with those of the NAATs.
Full Text
https://www.degruyter.com/doi/10.1515/cclm-2016-0130
DOI
10.1515/cclm-2016-0130
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyon Suk(김현숙) ORCID logo https://orcid.org/0000-0001-5662-7740
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/161249
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links