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Role of α-Actinin 2 in Cytoadherence and Cytotoxicity of Trichomonas vaginalis

 Hye-Yeon Lee  ;  Juri Kim  ;  Soon-Jung Park 
 Journal of Microbiology and Biotechnology, Vol.27(10) : 1844-1854, 2017 
Journal Title
 Journal of Microbiology and Biotechnology 
Issue Date
Actinin/genetics ; Actinin/physiology* ; Antigens, Protozoan/genetics ; Antigens, Protozoan/physiology ; Carrier Proteins/genetics ; Carrier Proteins/physiology ; Cell Line ; Epithelial Cells ; Female ; Gene Expression Regulation ; Humans ; Recombinant Proteins ; Trichomonas Infections/immunology ; Trichomonas vaginalis/genetics ; Trichomonas vaginalis/immunology ; Trichomonas vaginalis/metabolism* ; Trophozoites ; Vagina ; Virulence Factors
Trichomonas vaginalis is a pathogen that triggers severe immune responses in hosts. T. vaginalis α-actinin 2 (Tvα-actinin 2) has been used to diagnose trichomoniasis. Tvα-actinin 2 was dissected into three parts; the N-terminal, central, and C-terminal portions of the protein (#1, #2, and #3, respectively). Western blot of these Tvα-actinin 2 proteins with pooled patients' sera indicated that #2 and #3, but not #1, reacted with those sera. Immunofluorescence assays of two different forms of T. vaginalis (trophozoites and amoeboid forms), using anti-Tvα-actinin 2 antibodies, showed localization of Tvα-actinin 2 close to the plasma membranes of the amoeboid form. Fractionation experiments indicated the presence of Tvα-actinin 2 in cytoplasmic, membrane, and secreted proteins of T. vaginalis. Binding of fluorescence-labeled Trichomonas to vaginal epithelial cells and prostate cells was decreased in the antibody blocking experiment using anti-Tvα-actinin 2 antibodies. Pretreatment of T. vaginalis with anti-rTvα-actinin 2 antibodies also resulted in reduction in its cytotoxicity. Flow cytometry, ligand-binding immunoblotting assay, and observation by fluorescence microscopy were used to detect the binding of recombinant Tvα-actinin 2 to human epithelial cell lines. Specifically, the truncated N-terminal portion of Tvα-actinin 2, Tvα-actinin 2 #1, was shown to bind directly to vaginal epithelial cells. These data suggest that α-actinin 2 is one of the virulence factors responsible for the pathogenesis of T. vaginalis by serving as an adhesin to the host cells.
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1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Ju Ri(김주리) ORCID logo https://orcid.org/0000-0001-8270-7584
Park, Soon Jung(박순정) ORCID logo https://orcid.org/0000-0002-0423-1944
Lee, Hye Yeon(이혜연)
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