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A Far-Red-Emitting Fluorescence Probe for Sensitive and Selective Detection of Peroxynitrite in Live Cells and Tissues

Authors
 Di Wu  ;  Jae-Chan Ryu  ;  Youn Wook Chung  ;  Dayoung Lee  ;  Ji-Hwan Ryu  ;  Joo-Heon Yoon  ;  Juyoung Yoon 
Citation
 ANALYTICAL CHEMISTRY, Vol.89(20) : 10924-10931, 2017 
Journal Title
 ANALYTICAL CHEMISTRY 
ISSN
 0003-2700 
Issue Date
2017
Abstract
In this study, the far-red-emitting fluorescence probe 1, containing a rhodamine derivative and a hydrazide reactive group, was developed for peroxynitrite detection and imaging. This probe, which is cell permeable and shows high sensitivity and selectivity in fluorometric detection of peroxynitrite over other ROS/RNS, was successfully utilized to detect exogenous and endogenous peroxynitrite in HeLa and RAW 264.7 cells, respectively. More importantly, 1 can also be used to detect endogenous peroxynitrite generated in Pseudomonas aeruginosa (PAO1)-infected mouse bone marrow-derived neutrophils. We anticipate that the new probe will serve as a powerful molecular imaging tool in investigations of the role(s) played by peroxynitrite in a variety of physiological and pathological contexts.
Full Text
https://pubs.acs.org/doi/10.1021/acs.analchem.7b02707
DOI
10.1021/acs.analchem.7b02707
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Ryu, Ji Hwan(유지환)
Yoon, Joo Heon(윤주헌)
Chung, Youn Wook(정연욱) ORCID logo https://orcid.org/0000-0002-4382-1410
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/161176
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