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Investigation of dermal toxicity of ionic liquids in monolayer-cultured skin cells and 3D reconstructed human skin models.

Authors
 Jee-hyun Hwang  ;  Hyeonji Park  ;  Dal Woong Choi  ;  Ki Taek Nam  ;  Kyung-Min Lim 
Citation
 Toxicology in Vitro, Vol.25(46) : 194-202, 2017 
Journal Title
 Toxicology in Vitro 
ISSN
 0887-2333 
Issue Date
2017
Keywords
3D skin model ; Bis(trifluoromethanesulfonyl)imide, TFSI ; Dermal toxicity ; Ionic liquid ; ROS
Abstract
Ionic liquids have gained increasing attention in the chemical industry as potential green substitutes for traditional solvents. However, little is known about toxicity of ionic liquids on the skin, a major exposure portal to toxic substances. Here, we evaluated dermal toxicity of ionic liquids using human keratinocyte and fibroblast cell line, 3D reconstructed human epidermis, and full-thickness model to investigate underlying mechanisms. Cytotoxicity of ionic liquids was evaluated for representative anions, [TFSI], [PF6], [BF4], and [DCA], as well as for cations, [EMIM], [BMPY], [TBA] and [Zn], in human keratinocyte cell line, HaCaT, and human dermal fibroblasts. In our results, significant cytotoxicity was induced by ionic liquids with [TFSI] in both cell lines. Notably, cytotoxicity of [TFSI] containing ionic liquids was comparable to xylene, a toxic conventional organic solvent. Fluorescent and flow cytometric analysis revealed that [TFSI]-exposed cells underwent necrotic cell death. Reactive oxygen species (ROS) was increased while the amount of glutathione was decreased by [TFSI] in dose-dependent manner, which was reversed by antioxidant, N-acetylcysteine. In 3D reconstructed human epidermis and full-thickness model, a single application of [TFSI] induced toxicity although it was minimal and largely limited to epidermal layer. Collectively, these results demonstrated potential dermal toxicity of ionic liquids.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/161140
DOI
10.1016/j.tiv.2017.09.025
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부)
Yonsei Authors
남기택(Nam, Ki Taek)
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Full Text
https://www.sciencedirect.com/science/article/pii/S0887233317302771
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