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Investigation of dermal toxicity of ionic liquids in monolayer-cultured skin cells and 3D reconstructed human skin models.
DC Field | Value | Language |
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dc.contributor.author | 남기택 | - |
dc.date.accessioned | 2018-07-20T08:23:17Z | - |
dc.date.available | 2018-07-20T08:23:17Z | - |
dc.date.issued | 2018 | - |
dc.identifier.issn | 0887-2333 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/161140 | - |
dc.description.abstract | Ionic liquids have gained increasing attention in the chemical industry as potential green substitutes for traditional solvents. However, little is known about toxicity of ionic liquids on the skin, a major exposure portal to toxic substances. Here, we evaluated dermal toxicity of ionic liquids using human keratinocyte and fibroblast cell line, 3D reconstructed human epidermis, and full-thickness model to investigate underlying mechanisms. Cytotoxicity of ionic liquids was evaluated for representative anions, [TFSI], [PF6], [BF4], and [DCA], as well as for cations, [EMIM], [BMPY], [TBA] and [Zn], in human keratinocyte cell line, HaCaT, and human dermal fibroblasts. In our results, significant cytotoxicity was induced by ionic liquids with [TFSI] in both cell lines. Notably, cytotoxicity of [TFSI] containing ionic liquids was comparable to xylene, a toxic conventional organic solvent. Fluorescent and flow cytometric analysis revealed that [TFSI]-exposed cells underwent necrotic cell death. Reactive oxygen species (ROS) was increased while the amount of glutathione was decreased by [TFSI] in dose-dependent manner, which was reversed by antioxidant, N-acetylcysteine. In 3D reconstructed human epidermis and full-thickness model, a single application of [TFSI] induced toxicity although it was minimal and largely limited to epidermal layer. Collectively, these results demonstrated potential dermal toxicity of ionic liquids. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Pergamon Press | - |
dc.relation.isPartOf | TOXICOLOGY IN VITRO | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Investigation of dermal toxicity of ionic liquids in monolayer-cultured skin cells and 3D reconstructed human skin models. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Life Science | - |
dc.contributor.googleauthor | Jee-hyun Hwang | - |
dc.contributor.googleauthor | Hyeonji Park | - |
dc.contributor.googleauthor | Dal Woong Choi | - |
dc.contributor.googleauthor | Ki Taek Nam | - |
dc.contributor.googleauthor | Kyung-Min Lim | - |
dc.identifier.doi | 10.1016/j.tiv.2017.09.025 | - |
dc.contributor.localId | A01243 | - |
dc.relation.journalcode | J02743 | - |
dc.identifier.eissn | 1879-3177 | - |
dc.identifier.pmid | 28958837 | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0887233317302771 | - |
dc.subject.keyword | 3D skin model | - |
dc.subject.keyword | Bis(trifluoromethanesulfonyl)imide, TFSI | - |
dc.subject.keyword | Dermal toxicity | - |
dc.subject.keyword | Ionic liquid | - |
dc.subject.keyword | ROS | - |
dc.contributor.alternativeName | Nam, Ki Taek | - |
dc.contributor.affiliatedAuthor | Nam, Ki Taek | - |
dc.contributor.affiliatedAuthor | 남기택 | - |
dc.citation.volume | 46 | - |
dc.citation.startPage | 194 | - |
dc.citation.endPage | 202 | - |
dc.identifier.bibliographicCitation | TOXICOLOGY IN VITRO, Vol.46 : 194-202, 2018 | - |
dc.identifier.rimsid | 61066 | - |
dc.type.rims | ART | - |
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